Bioactive microfluidic paper device for pesticide determination in waters.

Autor: Fernández-Ramos MD; Department of Analytical Chemistry, Campus Fuentenueva, Faculty of Sciences, 18071, University of Granada, Spain; Unit of Excellence in Chemistry applied to Biomedicine and the Environment of the University of Granada, Spain. Electronic address: mdframos@ugr.es., Ogunneye AL; Department of Chemical Sciences, Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria., Babarinde NAA; Department of Chemical Sciences, Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria., Erenas MM; Department of Analytical Chemistry, Campus Fuentenueva, Faculty of Sciences, 18071, University of Granada, Spain; Unit of Excellence in Chemistry applied to Biomedicine and the Environment of the University of Granada, Spain., Capitán-Vallvey LF; Department of Analytical Chemistry, Campus Fuentenueva, Faculty of Sciences, 18071, University of Granada, Spain; Unit of Excellence in Chemistry applied to Biomedicine and the Environment of the University of Granada, Spain.
Jazyk: angličtina
Zdroj: Talanta [Talanta] 2020 Oct 01; Vol. 218, pp. 121108. Date of Electronic Publication: 2020 May 11.
DOI: 10.1016/j.talanta.2020.121108
Abstrakt: This work presents a new optical microfluidic paper biosensor for the detection of organophosphate pesticides and carbamate pesticides. The assay strip is composed of a paper support (1 × 17.6 mm) onto which acetylcholine esterase (AChE) and acetylcholine chloride (AChCl) are deposited, in such a way that there is a small hole between them that ensures that they only come into contact in the reaction zone when they are carried by a solution of the sample by lateral flow to the reaction zone containing bromocresol purple (BCP) as the pH indicator, immobilized by sol-gel. The sensor operates at room temperature and the rate of the inhibited reaction serves as an analytical signal, which is measured using a camera by quantifying the appropriate colour coordinate. Calibration curves were obtained for chlorpyrifos and carbaryl, with a useful concentration range from 0.24 to 20 μg L -1 for carbaryl and from 2.00 to 45 μg L -1 for chlorpyrifos. The detection limits were 0.24 and 2.00 μg L -1 , respectively, and with reproducibility around 4.2-5.5%. The method was applied to the determination of pesticides in different water samples, with no sample preparation.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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