Generation of a spinal muscular atrophy type III patient-specific induced pluripotent stem cell line ICGi003-A.

Autor:	Ovechkina VS; Federal Research Center Institute of Cytology and Genetics, Novosibirsk, Russia; E.N. Meshalkin National Medical Research Centre, Ministry of Healthcare of Russian Federation, Novosibirsk, Russia; National Research University Novosibirsk State University, Novosibirsk, Russia., Maretina MA; D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, Saint Petersburg, Russia., Egorova AA; D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, Saint Petersburg, Russia., Baranov VS; D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, Saint Petersburg, Russia., Kiselev AV; D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, Saint Petersburg, Russia., Zakian SM; Federal Research Center Institute of Cytology and Genetics, Novosibirsk, Russia; E.N. Meshalkin National Medical Research Centre, Ministry of Healthcare of Russian Federation, Novosibirsk, Russia; National Research University Novosibirsk State University, Novosibirsk, Russia; Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia., Valetdinova KR; Federal Research Center Institute of Cytology and Genetics, Novosibirsk, Russia; E.N. Meshalkin National Medical Research Centre, Ministry of Healthcare of Russian Federation, Novosibirsk, Russia; National Research University Novosibirsk State University, Novosibirsk, Russia. Electronic address: valetdinova@bionet.nsc.ru.
Jazyk:	angličtina
Zdroj:	Stem cell research [Stem Cell Res] 2020 Oct; Vol. 48, pp. 101938. Date of Electronic Publication: 2020 Aug 03.
DOI:	10.1016/j.scr.2020.101938
Abstrakt:	Spinal muscular atrophy (SMA) is a genetic disease, which characterized by the degeneration of motor neurons in the spinal cord and further striated muscle atrophy. The research of the processes in diseased neurons is complicated due to the impossibility of obtaining them safely from patients. Thus, we generated SMA type III induced pluripotent stem cell lines via using non-integrated episomal plasmid vectors. The resulting cell line expresses the major pluripotency markers and can differentiate in vitro into derivatives of three germ layers. The iPSC line can be used for further studies by providing in vitro the relevant cell types. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze:	MEDLINE
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