Statistical optimization of cultural variables for enzymatic degradation of aflatoxin B 1 by Panus neostrigosus.

Autor: Söylemez T; Savaş Kubaş Anatolian High School, 26050, Eskişehir, Turkey. Electronic address: tuncaysoylemez1@gmail.com., Yamaç M; Eskisehir Osmangazi University, Faculty of Science and Letters, Department of Biology, 26480, Eskisehir, Turkey., Yıldız Z; Eskisehir Osmangazi University, Faculty of Science and Letters, Department of Statistics, 26480, Eskisehir, Turkey.
Jazyk: angličtina
Zdroj: Toxicon : official journal of the International Society on Toxinology [Toxicon] 2020 Oct 30; Vol. 186, pp. 141-150. Date of Electronic Publication: 2020 Aug 11.
DOI: 10.1016/j.toxicon.2020.08.003
Abstrakt: The aim of this study is to determine the best aflatoxin B 1 degradation conditions which was optimized using a combination of the Plackett-Burman and Box-Behnken methods with Panus neostrigosus culture filtrate. Panus neostrigosus was grown in a modified Kirk Broth medium to determine optimal degradation conditions. As a result, aflatoxin B 1 was degraded under varying culture conditions. The Plackett-Burman method was designed after sixteen different experiments with fifteen variables. The three most effective variables (Sucrose, yeast extract, wheat bran) were chosen for the Box-Behnken methodology. The aflatoxin B 1 degradation rate was 49% in just 1 h exposure to culture filtrate which was obtained under optimal growth conditions; (g-ml/L) sucrose 10, yeast extract 3, wheat bran 3, soytone 5, KH 2 PO 4 2, MgSO 4 .7H 2 O 0.5, CaCl 2 .H 2 O 0.1, ammonium tartrate 2, trace element solution 10; 28 °C of incubation temperature, medium pH 5, 7.5% inoculum rate, 125 rpm of agitation speed, and a twelve-day incubation period. The SDS-PAGE studies show that the enzyme responsible for AFB 1 degradation has 38 kDa molecular weight and has no laccase or MnP activity. To the best of our knowledge, this is the first report for AFB 1 degradation by Panus neostrigosus.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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