Evaluation of the effect of some medicinal plants on cultured Trichomonas Vaginalis.
| Autor: | Selim MA; Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Zagazig, Egypt. dr.moniraseliem@gmail.com., Fawzy EM; Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Zagazig, Egypt. e.magdy_83@hotmail.com., Abd El-Rahman EM; Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Zagazig, Egypt. emanmostafa81@gmail.com., Abdel Hady RS; Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Zagazig, Egypt. dr.reem_safaa@yahoo.com., Badr MS; Department of Obstetrics and Gynecology, Faculty of Medicine, Zagazig University, Zagazig, Egypt. 2362044@gmail.com., Abdel Hamed EF; Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Zagazig, Egypt. enas_refae1983@yahoo.com. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Journal of infection in developing countries [J Infect Dev Ctries] 2020 Jul 31; Vol. 14 (7), pp. 793-799. Date of Electronic Publication: 2020 Jul 31. |
| DOI: | 10.3855/jidc.11580 |
| Abstrakt: | Introduction: Trichomoniasis is a worldwide sexually transmitted disease caused by Trichomonas vaginalis. It inflicts severe complications to the human genitourinary system. The devastating negative effects and the emergence of resistance to common medication impose the search for safer and effective alternatives. This research aimed to investigate the effect of the Allium sativum, Nigella sativa crude extracts (NsCE) and the combination between their most effective doses with metronidazole. Methodology: Vaginal swabs were obtained from symptomatic patients, and cultured on Diamond's medium. Assessment of various concentrations of these herbs at different follow-up periods was done by counting the number of dead T. vaginalis trophozoites using the hemocytometer and trypan blue staining. Transmission electron microscope study was done. Results: NsCE 9 mg/mL yielded the highest lethal effect on T. vaginalis trophozoites after 72 hours, compared with metronidazole. Combination of NsCE 9 mg/mL and metronidazole 50 µg/mL gave the best result. Additionally, Tomex90 µg/mL, represents a tolerable effect after 72 hours, but metronidazole 100 µg/mL still has higher effect. These results were confirmed by the ultrastructural changes observed in T. vaginalis trophozoites, signifying severe damage of nucleus and cytoplasm with large vacuolization and cell membrane defects. Conclusions: NsCE is a promising anti-Trichomonas especially its combination with metronidazole which showed a high synergistic effect. Competing Interests: No Conflict of Interest is declared (Copyright (c) 2020 Monira Abdel Wahab Selim, Eman Magdy Fawzy, Eman Mostafa Abd El-Rahman, Reem Safaa Eldeen Abdel Hady, Mohamed Samir Badr, Enas Fakhry Abdel Hamed.) |
| Databáze: | MEDLINE |
| Externí odkaz: |
|