Inducible nitric oxide synthase is required for epidermal permeability barrier homeostasis in mice.

Autor: Dang E; Dermatology Service Veterans Affairs Medical Center, Department of Dermatology, University of California, San Francisco, CA, USA., Man G; Dermatology Service Veterans Affairs Medical Center, Department of Dermatology, University of California, San Francisco, CA, USA., Zhang J; Dermatology Service Veterans Affairs Medical Center, Department of Dermatology, University of California, San Francisco, CA, USA., Lee D; Dermatology Service Veterans Affairs Medical Center, Department of Dermatology, University of California, San Francisco, CA, USA., Mauro TM; Dermatology Service Veterans Affairs Medical Center, Department of Dermatology, University of California, San Francisco, CA, USA., Elias PM; Dermatology Service Veterans Affairs Medical Center, Department of Dermatology, University of California, San Francisco, CA, USA., Man MQ; Dermatology Service Veterans Affairs Medical Center, Department of Dermatology, University of California, San Francisco, CA, USA.
Jazyk: angličtina
Zdroj: Experimental dermatology [Exp Dermatol] 2020 Oct; Vol. 29 (10), pp. 1027-1032.
DOI: 10.1111/exd.14176
Abstrakt: Nitric oxide (NO) regulates a variety of epidermal functions, including epidermal proliferation, differentiation and cutaneous wound healing. However, whether nitric oxide (NO) and its synthetic enzymes regulate epidermal permeability barrier homeostasis is not clear. In the present study, we employed inducible nitric oxide synthase (iNOS) KO mice to explore the role of iNOS in epidermal permeability barrier homeostasis. Our results showed that iNOS mice displayed a comparable levels of basal transepidermal water loss rates, stratum corneum hydration and skin surface pH to their wild-type mice, but epidermal permeability barrier recovery was significantly delayed both 2 and 4 hours after acute barrier disruption by tape stripping. In parallel, expression levels of mRNA for epidermal differentiation-related proteins and lipid synthetic enzymes were lower in iNOS KO mice versus wild-type controls. Topical applications of two structurally unrelated NO donors to iNOS KO mice improved permeability barrier recovery kinetics and upregulated expression levels of mRNA for epidermal differentiation-related proteins and lipid synthetic enzymes. Together, these results indicate that iNOS and its product regulate epidermal permeability barrier homeostasis in mice.
(Published 2020. This article is a U.S. Government work and is in the public domain in the USA.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje