Hydrogel-mediated co-transplantation of retinal pigmented epithelium and photoreceptors restores vision in an animal model of advanced retinal degeneration.

Autor: Mitrousis N; Institute of Biomedical Engineering, University of Toronto, Toronto, ON, M5S 3G9, Canada; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, M5S 3E1, Canada., Hacibekiroglu S; Lunenfeld-Tanenbaum Research Institute, Mt Sinai Hospital, Toronto, ON, M5G 1X5, Canada; Institute of Medical Science, University of Toronto, Toronto, ON, M5S 1A8, Canada., Ho MT; Institute of Biomedical Engineering, University of Toronto, Toronto, ON, M5S 3G9, Canada; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, M5S 3E1, Canada., Sauvé Y; Department of Ophthalmology and Visual Sciences, University of Alberta, Edmonton, AB, T6G 2R7, Canada., Nagy A; Lunenfeld-Tanenbaum Research Institute, Mt Sinai Hospital, Toronto, ON, M5G 1X5, Canada; Institute of Medical Science, University of Toronto, Toronto, ON, M5S 1A8, Canada; Department of Obstetrics and Gynaecology, University of Toronto, Toronto, ON, M5G 1E2, Canada., van der Kooy D; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, M5S 3E1, Canada; Institute of Medical Science, University of Toronto, Toronto, ON, M5S 1A8, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, M5S 1A8, Canada., Shoichet MS; Institute of Biomedical Engineering, University of Toronto, Toronto, ON, M5S 3G9, Canada; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, M5S 3E1, Canada; Institute of Medical Science, University of Toronto, Toronto, ON, M5S 1A8, Canada; Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, M5S 3E5, Canada; Department of Chemistry, University of Toronto, Toronto, ON, M5S 3H6, Canada. Electronic address: molly.shoichet@utoronto.ca.
Jazyk: angličtina
Zdroj: Biomaterials [Biomaterials] 2020 Oct; Vol. 257, pp. 120233. Date of Electronic Publication: 2020 Jul 30.
DOI: 10.1016/j.biomaterials.2020.120233
Abstrakt: We demonstrate a novel approach to reverse advanced stages of blindness using hydrogel-mediated delivery of retinal pigmented epithelium (RPE) and photoreceptors directly to the degenerated retina of blind mice. With sodium iodate (NaIO 3 ) injections in mice, both RPE and photoreceptors degenerate, resulting in complete blindness and recapitulating the advanced retinal degeneration that is often observed in humans. We observed vision restoration only with co-transplantation of RPE and photoreceptors in a hyaluronic acid-based hydrogel, and not with transplantation of each cell type alone as determined with optokinetic head tracking and light avoidance assays. Both RPE and photoreceptors survived significantly better when co-transplanted than in their respective single cell type controls. While others have pursued transplantation of one of either RPE or photoreceptors, we demonstrate the importance of transplanting both cell types with a minimally-invasive hydrogel for vision repair in a degenerative disease model of the retina.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: