The pneumococcal two-component system SirRH is linked to enhanced intracellular survival of Streptococcus pneumoniae in influenza-infected pulmonary cells.

Autor: Reinoso-Vizcaíno NM; Departamento de Bioquímica Clínica-CIBICI (CONICET), Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina., Cian MB; Departamento de Bioquímica Clínica-CIBICI (CONICET), Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina.; Department of Microbiology and Immunology, Health Sciences Center, University of Oklahoma, Oklahoma, United States of America., Cortes PR; Departamento de Bioquímica Clínica-CIBICI (CONICET), Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina., Olivero NB; Departamento de Bioquímica Clínica-CIBICI (CONICET), Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina., Hernandez-Morfa M; Departamento de Bioquímica Clínica-CIBICI (CONICET), Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina., Piñas GE; Departamento de Bioquímica Clínica-CIBICI (CONICET), Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina.; School of Biological Sciences, University of Utah, Salt Lake City, Utah, United States of America., Badapanda C; Bioinformatics Division, Xcelris Labs Limited, Ahmedabad, India., Rathore A; Bioinformatics Division, Xcelris Labs Limited, Ahmedabad, India., Perez DR; Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, Georgia, United States of America., Echenique J; Departamento de Bioquímica Clínica-CIBICI (CONICET), Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
Jazyk: angličtina
Zdroj: PLoS pathogens [PLoS Pathog] 2020 Aug 13; Vol. 16 (8), pp. e1008761. Date of Electronic Publication: 2020 Aug 13 (Print Publication: 2020).
DOI: 10.1371/journal.ppat.1008761
Abstrakt: The virus-bacterial synergism implicated in secondary bacterial infections caused by Streptococcus pneumoniae following infection with epidemic or pandemic influenza A virus (IAV) is well documented. However, the molecular mechanisms behind such synergism remain largely ill-defined. In pneumocytes infected with influenza A virus, subsequent infection with S. pneumoniae leads to enhanced pneumococcal intracellular survival. The pneumococcal two-component system SirRH appears essential for such enhanced survival. Through comparative transcriptomic analysis between the ΔsirR and wt strains, a list of 179 differentially expressed genes was defined. Among those, the clpL protein chaperone gene and the psaB Mn+2 transporter gene, which are involved in the stress response, are important in enhancing S. pneumoniae survival in influenza-infected cells. The ΔsirR, ΔclpL and ΔpsaB deletion mutants display increased susceptibility to acidic and oxidative stress and no enhancement of intracellular survival in IAV-infected pneumocyte cells. These results suggest that the SirRH two-component system senses IAV-induced stress conditions and controls adaptive responses that allow survival of S. pneumoniae in IAV-infected pneumocytes.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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