Development of standardized method for the quantification of azo dyes by UV-Vis in binary mixtures.

Autor: García-González A; Tecnológico Nacional de México/Instituto Tecnológico de Toluca, Av. Tecnológico S/n. Colonia Agrícola Bellavista, Metepec, Estado de México, C.P. 52149, Mexico., Zavala-Arce RE; Tecnológico Nacional de México/Instituto Tecnológico de Toluca, Av. Tecnológico S/n. Colonia Agrícola Bellavista, Metepec, Estado de México, C.P. 52149, Mexico. Electronic address: rzavalaa@toluca.tecnm.mx., Avila-Pérez P; Tecnológico Nacional de México/Instituto Tecnológico de Toluca, Av. Tecnológico S/n. Colonia Agrícola Bellavista, Metepec, Estado de México, C.P. 52149, Mexico. Electronic address: pavilap@toluca.tecnm.mx., Jiménez-Núñez ML; Tecnológico Nacional de México/Instituto Tecnológico de Toluca, Av. Tecnológico S/n. Colonia Agrícola Bellavista, Metepec, Estado de México, C.P. 52149, Mexico., García-Gaitán B; Tecnológico Nacional de México/Instituto Tecnológico de Toluca, Av. Tecnológico S/n. Colonia Agrícola Bellavista, Metepec, Estado de México, C.P. 52149, Mexico., García-Rivas JL; Tecnológico Nacional de México/Instituto Tecnológico de Toluca, Av. Tecnológico S/n. Colonia Agrícola Bellavista, Metepec, Estado de México, C.P. 52149, Mexico.
Jazyk: angličtina
Zdroj: Analytical biochemistry [Anal Biochem] 2020 Nov 01; Vol. 608, pp. 113897. Date of Electronic Publication: 2020 Aug 08.
DOI: 10.1016/j.ab.2020.113897
Abstrakt: The azo dyes, Yellow 5 (Y5), Red 2 (R2) and Blue 1 (B1), quantified in solutions and in mixtures of binary dyes, were studied by means of UV-Vis spectroscopy. In this work was used a CIE algorithm developed in Visual Basic for Applications (VBA). The CIE algorithm is based on the tristimulus chromaticity diagram, as an alternative to the shielding effect that arises in dye mixtures, and it can also be applied to complex quantification methods such as HPLC (High Performance Liquid Chromatography). The results obtained through of the algorithm, showed a higher accuracy from 97 to 99% in relation with similar UV-Vis quantification methods. In contrast, linear methods only managed to reach an accuracy from 78 to 98%. Additionally, the algorithm yielded significant similar values to the UHPLC reference method. The results showed that the method CIE algorithm was accessible and reliable to quantify binary mixtures of the dyes used which suggests the possibility to apply this method on other dyes, within the limits of quantification obtained in this study (0.076-24.56 mg/L) and the pH values from 2 to 10.
(Copyright © 2020 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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