Lignin isolated from Caesalpinia pulcherrima leaves has antioxidant, antifungal and immunostimulatory activities.

Autor: de Melo CML; Department of Antibiotics, Biosciences Centre, Federal University of Pernambuco (UFPE), Recife, PE, Brazil. Electronic address: cristiane.moutinho@ufpe.br., da Cruz Filho IJ; Department of Antibiotics, Biosciences Centre, Federal University of Pernambuco (UFPE), Recife, PE, Brazil., de Sousa GF; Department of Antibiotics, Biosciences Centre, Federal University of Pernambuco (UFPE), Recife, PE, Brazil., de Souza Silva GA; Department of Antibiotics, Biosciences Centre, Federal University of Pernambuco (UFPE), Recife, PE, Brazil., do Nascimento Santos DKD; Department of Antibiotics, Biosciences Centre, Federal University of Pernambuco (UFPE), Recife, PE, Brazil., da Silva RS; Department of Antibiotics, Biosciences Centre, Federal University of Pernambuco (UFPE), Recife, PE, Brazil., de Sousa BR; Laboratory of Medical Mycology, Department of Mycology, Federal University of Pernambuco (UFPE), Recife, PE, Brazil., de Lima Neto RG; Laboratory of Medical Mycology, Department of Mycology, Federal University of Pernambuco (UFPE), Recife, PE, Brazil., do Carmo Alves de Lima M; Department of Antibiotics, Biosciences Centre, Federal University of Pernambuco (UFPE), Recife, PE, Brazil., de Moraes Rocha GJ; Brazilian Biorenewables National Laboratory (LNBR), Brazilian Center for Research in Energy and Materials-CNPEM, Campinas, SP, Brazil.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2020 Nov 01; Vol. 162, pp. 1725-1733. Date of Electronic Publication: 2020 Aug 07.
DOI: 10.1016/j.ijbiomac.2020.08.003
Abstrakt: Caesalpinia pulcherrima is a shrub with worldwide distribution used as an ornamental plant. In this study, we extracted a lignin from the C. pulcherrima leaves and investigated its biological functions. The lignin was characterized by FT-IR, UV-Vis, GPC, TGA and nuclear magnetic resonance ( 1 H and 13 C). The antioxidant activity was evaluated using phosphomolybdenum complexation methods (TAA), sequestration of DPPH and ABTS radicals, reducing power, formation of nitrite radical and iron chelating activity (Fe 2 + ). Antifungal activity was made using Candida spp., Aspergillus spp. and Cryptococcus neoformans strains. Cytotoxicity, oxidative stress, and cytokine production were performed using mouse splenocytes. The lignin showed maximal UV-Vis at ~280 nm, 22.27 L/g·cm of absorptivity and, 2,503 kDa of molecular weight. Phenolic compounds (41.33 ± 0.65 mg GAE/g) and indications of a guaiacyl-syringyl-hydroxyphenyl (GSH)-type composition were found. Antioxidant activities of lignin to TAA (40±1.2%) and to DPPH (16.9±0.2%) was high and showed antifungal potential, especially against Candida spp. (IC 50  = 31.3 μg/mL) and C. neoformans (15.6 μg/mL). In mouse splenocytes, the lignin was not cytotoxic and stimulated the cell proliferation and cytokine release. These results indicate that C. pulcherrima lignin has the potential to be used as antifungal and immunostimulant compound.
Competing Interests: Declaration of competing interest The authors have no conflict of interest with this work.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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