Altered Regulatory B Cell Subsets in Children with Type 1 Diabetes Mellitus.

Autor: El-Mokhtar MA; Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Egypt., Elsherbiny NM; Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Egypt., Sayed D; Department of Clinical Pathology, South Egypt Cancer Institute, Assiut University, Egypt., Raafat DM; Department of Pediatrics, Faculty of Medicine, Assiut University, Egypt., Askar E; Department of Pediatrics, Faculty of Medicine, Assiut University, Egypt., Hussein A; Department of Pediatrics, Faculty of Medicine, Assiut University, Egypt., Abdel-Malek MAY; Assiut University Children's Hospital, Faculty of Medicine, Assiut University, Egypt., Shalaby AM; Department of Pediatrics, Faculty of Medicine, Assiut University, Egypt.
Jazyk: angličtina
Zdroj: Journal of immunology research [J Immunol Res] 2020 Jul 21; Vol. 2020, pp. 8935694. Date of Electronic Publication: 2020 Jul 21 (Print Publication: 2020).
DOI: 10.1155/2020/8935694
Abstrakt: B regulatory cells (Breg) refer to characteristic subsets of B cells that generally exert anti-inflammatory functions and maintain peripheral tolerance mainly through their ability to secrete interleukin-10 (IL10). Dysregulation in the function of Breg cells was reported in several autoimmune diseases. However, the relation between Breg and children with type 1 diabetes (T1D) is poorly understood. Thus, this study is aimed at determining whether Breg cells play a role in T1D in children or not, so we hypothesized that an altered phenotype of B cell subsets is associated with T1D in children. Children with T1D ( n = 29) and control children with normal blood glucose levels ( n = 14) were recruited. The percentages of different circulating IL10-producing Breg subsets, including B10, immature transitional, and plasmablasts were determined using flow cytometry analysis. Furthermore, the association between different IL10-producing B cells and patient parameters was investigated. The percentage of circulating IL10 + CD24 hi CD27 + (B10) and IL10 + CD24 hi CD38 hi (immature transitional) subsets of Breg cells was significantly lower in T1D patients than in healthy controls. Moreover, these cells were also negatively correlated with fasting blood glucose and HbA1c levels. Breg cells did not correlate with autoantibody levels in the serum. These findings suggest that certain Breg subsets are numerically deficient in children with T1D. This alteration in frequency is associated with deficient islet function and glycemia. These findings suggest that Breg cells may be involved in the loss of auto-tolerance and consequent destruction of pancreatic cells and could, therefore, be a potential target for immunotherapy.
Competing Interests: The authors declare no conflict of interest.
(Copyright © 2020 Mohamed A. El-Mokhtar et al.)
Databáze: MEDLINE
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