Abyssinone V-4' Methyl Ether, a Flavanone Isolated from Erythrina droogmansiana , Exhibits Cytotoxic Effects on Human Breast Cancer Cells by Induction of Apoptosis and Suppression of Invasion.

Autor: Zingue S; Department of Life and Earth Sciences, Higher Teachers' Training College, University of Maroua, P.O. Box 55, Maroua, Cameroon.; Department of Pharmaceutical Sciences, Health Sciences Centre, Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil.; Department of Chemical Sciences, Faculty of Science, University of Johannesburg, Doornfontein 2028, South Africa., Gbaweng Yaya AJ; Department of Chemistry, Faculty of Science, University of Ngaoundere, P.O. Box 454, Ngaoundere, Cameroon., Cisilotto J; Department of Pharmaceutical Sciences, Health Sciences Centre, Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil., Kenmogne LV; Department of Animal Biology and Physiology, Faculty of Science, University of Yaoundé 1, P.O. Box 812, Yaoundé, Cameroon., Talla E; Department of Chemistry, Faculty of Science, University of Ngaoundere, P.O. Box 454, Ngaoundere, Cameroon., Bishayee A; Lake Erie College of Osteopathic Medicine, Bradenton, FL 34211, USA., Njamen D; Department of Chemical Sciences, Faculty of Science, University of Johannesburg, Doornfontein 2028, South Africa.; Department of Animal Biology and Physiology, Faculty of Science, University of Yaoundé 1, P.O. Box 812, Yaoundé, Cameroon., Creczynski-Pasa TB; Department of Pharmaceutical Sciences, Health Sciences Centre, Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil., Ndinteh DT; Department of Chemical Sciences, Faculty of Science, University of Johannesburg, Doornfontein 2028, South Africa.
Jazyk: angličtina
Zdroj: Evidence-based complementary and alternative medicine : eCAM [Evid Based Complement Alternat Med] 2020 Jul 22; Vol. 2020, pp. 6454853. Date of Electronic Publication: 2020 Jul 22 (Print Publication: 2020).
DOI: 10.1155/2020/6454853
Abstrakt: Abyssinone V-4' methyl ether (AVME) isolated from Erythrina droogmansiana was recently reported to exhibit anti-mammary tumor effect in mice. The present work was therefore aimed at elucidating its cellular and molecular mechanisms. To achieve our goal, the cytotoxicity of AVME against tumoral and non-tumoral cell lines was evaluated by resazurin reduction test; flow cytometry allowed us to evaluate the cell cycle and mechanisms of cell death; the mitochondrial transmembrane potential, reactive oxygen species (ROS) levels, and caspase activities as well as apoptosis-regulatory proteins (Bcl-2 and Bcl-XL) were measured in MDA-MB-231 cells. Further, the antimetastatic potential of AVME was evaluated by invasion assay. AVME exhibited cytotoxic effects in all tested tumor cell lines and induced a significant increase in the percentage of MDA-MB-231 cells at G2/M and S phases of the cell cycle in a concentration-dependent manner. AVME also induced apoptosis in MDA-MB-231 cells, which was accompanied by the activation of caspase-3 and caspase-9 and downregulation of Bcl-2 and Bcl-XL proteins. Moreover, AVME suppressed cancer cell invasion by the inhibition of the metalloproteinase-9 activity. Findings from this study suggest that AVME has anti-breast cancer activities expressed through mitochondrial proapoptotic pathway including impairment of aggressive behaviors of breast cancer cells.
Competing Interests: The authors declare no conflicts of interest.
(Copyright © 2020 Stéphane Zingue et al.)
Databáze: MEDLINE
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