Clinical metagenomic sequencing for diagnosis of pulmonary tuberculosis.

Autor: Shi CL; The Affiliated Infectious Hospital of Soochow University, 10 Guangqian Road, Suzhou, Suzhou, Jiangsu 215131, China; The Fifth People's Hospital of Suzhou, Suzhou, China., Han P; Genskey Medical Technology Co., Ltd, Beijing, China., Tang PJ; The Affiliated Infectious Hospital of Soochow University, 10 Guangqian Road, Suzhou, Suzhou, Jiangsu 215131, China; The Fifth People's Hospital of Suzhou, Suzhou, China., Chen MM; Genskey Medical Technology Co., Ltd, Beijing, China., Ye ZJ; The Affiliated Infectious Hospital of Soochow University, 10 Guangqian Road, Suzhou, Suzhou, Jiangsu 215131, China; The Fifth People's Hospital of Suzhou, Suzhou, China., Wu MY; The Affiliated Infectious Hospital of Soochow University, 10 Guangqian Road, Suzhou, Suzhou, Jiangsu 215131, China; The Fifth People's Hospital of Suzhou, Suzhou, China., Shen J; The Affiliated Infectious Hospital of Soochow University, 10 Guangqian Road, Suzhou, Suzhou, Jiangsu 215131, China; The Fifth People's Hospital of Suzhou, Suzhou, China., Wu HY; The Affiliated Infectious Hospital of Soochow University, 10 Guangqian Road, Suzhou, Suzhou, Jiangsu 215131, China; The Fifth People's Hospital of Suzhou, Suzhou, China., Tan ZQ; The Affiliated Infectious Hospital of Soochow University, 10 Guangqian Road, Suzhou, Suzhou, Jiangsu 215131, China; The Fifth People's Hospital of Suzhou, Suzhou, China., Yu X; The Affiliated Infectious Hospital of Soochow University, 10 Guangqian Road, Suzhou, Suzhou, Jiangsu 215131, China; The Fifth People's Hospital of Suzhou, Suzhou, China. Electronic address: xinxin810104@126.com., Rao GH; Genskey Medical Technology Co., Ltd, Beijing, China. Electronic address: gh.rao@genskey.com., Zhang JP; The Affiliated Infectious Hospital of Soochow University, 10 Guangqian Road, Suzhou, Suzhou, Jiangsu 215131, China; The Fifth People's Hospital of Suzhou, Suzhou, China. Electronic address: 906168980@qq.com.
Jazyk: angličtina
Zdroj: The Journal of infection [J Infect] 2020 Oct; Vol. 81 (4), pp. 567-574. Date of Electronic Publication: 2020 Aug 05.
DOI: 10.1016/j.jinf.2020.08.004
Abstrakt: Objectives: The aim of this study is to investigate the clinical usefulness of metagenomic Next-generation sequencing (mNGS) on bronchoalveolar lavage fluid (BALF) samples to discriminate pulmonary tuberculosis (PTB) from Non-TB community-acquired pneumonia (CAP) in PTB suspects.
Methods: We investigate the performance of mNGS on BALF samples from 110 PTB suspects, in comparison with conventional microbiological testing (solid media culture, acid-fast bacilli staining (AFS), Xpert) of BALF or sputum samples and final clinical diagnosis.
Results: We finally clinically diagnosed 48 cases of pulmonary tuberculosis patients and 62 cases of non-tuberculosis patients. Comparing to the final clinical diagnosis, mNGS produced a sensitivity of 47.92%, which was similar to that of Xpert (45.83%) and culture (46.81%), but much higher than that of AFS (29.17%) for TB diagnosis in BALF samples. Apart from detecting Mycobacterium tuberculosis, mNGS also identified mixed infections in PTB patients, including 3 fungal cases and 1 bacteria case. Meanwhile, mNGS efficiently identified 14 of 22 (63.63%) cases of non-tuberculous mycobacteria (NTM), 7 cases of fungi, 1 case of viral infection, and other common bacterial pathogens in Non-PTB group. Finally, mNGS identified 67.23% infection cases within 3 days, while the conventional methods identified 49.58% infection cases for over 90 days.
Conclusion: Our data show that mNGS of BALF represents a potentially effective tool for the rapid diagnosis of PTB suspects.
Competing Interests: Declaration of Competing Interest We declare that we do not have any commercial or associative interest that represents a conflict of interest in connection with the work submitted.
(Copyright © 2020 The British Infection Association. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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