Full-genome analysis and pathogenicity of a genetically distinct Russian PRRSV-1 Tyu16 strain.

Autor: Yuzhakov AG; Federal State Budget Scientific Institution 'Federal Scientific Centre VIEV' (FSC VIEV), Moscow, Russia. Electronic address: anton_oskol@mail.ru., Raev SA; Federal State Budget Scientific Institution 'Federal Scientific Centre VIEV' (FSC VIEV), Moscow, Russia. Electronic address: raevsergey33@gmail.com., Shchetinin AM; N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology, Moscow, Russia. Electronic address: shchetinin.alexey@yandex.ru., Gushchin VA; N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology, Moscow, Russia. Electronic address: wowaniada@gmail.com., Alekseev KP; Federal State Budget Scientific Institution 'Federal Scientific Centre VIEV' (FSC VIEV), Moscow, Russia. Electronic address: kalekseev@hotmail.com., Stafford VV; Federal State Budget Scientific Institution 'Federal Scientific Centre VIEV' (FSC VIEV), Moscow, Russia. Electronic address: stafford.v.v@gmail.com., Komina AK; Federal State Budget Scientific Institution 'Federal Scientific Centre VIEV' (FSC VIEV), Moscow, Russia. Electronic address: komina.a.k@yandex.ru., Zaberezhny AD; Federal State Budget Scientific Institution 'Federal Scientific Centre VIEV' (FSC VIEV), Moscow, Russia. Electronic address: zaberezhny@mail.ru., Gulyukin AM; Federal State Budget Scientific Institution 'Federal Scientific Centre VIEV' (FSC VIEV), Moscow, Russia. Electronic address: admin@viev.ru., Aliper TI; Federal State Budget Scientific Institution 'Federal Scientific Centre VIEV' (FSC VIEV), Moscow, Russia. Electronic address: aliper@narvac.com.
Jazyk: angličtina
Zdroj: Veterinary microbiology [Vet Microbiol] 2020 Aug; Vol. 247, pp. 108784. Date of Electronic Publication: 2020 Jul 03.
DOI: 10.1016/j.vetmic.2020.108784
Abstrakt: Porcine reproductive and respiratory syndrome virus-1 (PRRSV-1) strains from Eastern Europe have a high diversity. All three known subtypes (1, 2, 3) of PRRSV-1 have been detected in Russia. There are two different groups of viruses belonging to the subtype 1: pan-European subtype 1 strains, and insufficiently studied Russian strains. The main objective of this study was to characterize the full genomic structure of the atypical Tyu16 strain of the Russian group subtype 1 PRRSV-1 and to assess its pathogenicity. Complete sequencing of the Tyu16 strain revealed that it did not belong to any existing subtype. Comparison of the whole genome sequence of the Tyu16 strain with that of PRRSV-1 prototype strains revealed 78.1 % (subtype 1 Lelystad), 78.1 % (subtype 2 WestSib13) and 77.7 % (subtype 3 Lena) nucleotide identity level, respectively. The coding sequence of different parts of the Tyu16 strain genome demonstrated a varying percentage identity to the different reference PRRSV-1 strains, which may indicate recombination events in its evolutionary history. We assume that among PRRSV-1 isolates, the Tyu16 is the closest relative to the common ancestor of PRRSV-1 and PRRSV-2. Low pathogenicity of the Tyu16 was demonstrated by experimental infection of 70-day-old piglets. Infected animals showed fever not exceeding 7 days, dyspnea in two out of five pigs and reduced weight gain. The virus shedding was undetectable and viremia was at low level.
Competing Interests: Declaration of Competing Interest The authors declare that they have no competing interests.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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