The Sphingosine Kinase 1 Inhibitor, PF543, Mitigates Pulmonary Fibrosis by Reducing Lung Epithelial Cell mtDNA Damage and Recruitment of Fibrogenic Monocytes.

Autor:	Cheresh P; Department of Medicine, Division of Pulmonary & Critical Care Medicine, Jesse Brown VA Medical Center, Chicago, IL 60611, USA.; Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA., Kim SJ; Department of Medicine, Division of Pulmonary & Critical Care Medicine, Jesse Brown VA Medical Center, Chicago, IL 60611, USA.; Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA., Huang LS; Department of Pharmacology and Regenerative Medicine, University of Illinois at Chicago, Chicago, IL 60612 USA., Watanabe S; Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA., Joshi N; Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA., Williams KJN; Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA., Chi M; Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA., Lu Z; Department of Medicine, Division of Pulmonary & Critical Care Medicine, Jesse Brown VA Medical Center, Chicago, IL 60611, USA.; Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA., Harijith A; Department of Pharmacology and Regenerative Medicine, University of Illinois at Chicago, Chicago, IL 60612 USA., Yeldandi A; Department of Pathology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA., Lam AP; Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA., Gottardi C; Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA., Misharin AV; Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA., Budinger GRS; Department of Medicine, Division of Pulmonary & Critical Care Medicine, Jesse Brown VA Medical Center, Chicago, IL 60611, USA.; Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA., Natarajan V; Department of Pharmacology and Regenerative Medicine, University of Illinois at Chicago, Chicago, IL 60612 USA.; Department of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA., Kamp DW; Department of Medicine, Division of Pulmonary & Critical Care Medicine, Jesse Brown VA Medical Center, Chicago, IL 60611, USA.; Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.
Jazyk:	angličtina
Zdroj:	International journal of molecular sciences [Int J Mol Sci] 2020 Aug 05; Vol. 21 (16). Date of Electronic Publication: 2020 Aug 05.
DOI:	10.3390/ijms21165595
Abstrakt:	Idiopathic pulmonary fibrosis (IPF) is a chronic disease for which novel approaches are urgently required. We reported increased sphingosine kinase 1 (SPHK1) in IPF lungs and that SPHK1 inhibition using genetic and pharmacologic approaches reduces murine bleomycin-induced pulmonary fibrosis. We determined whether PF543, a specific SPHK1 inhibitor post bleomycin or asbestos challenge mitigates lung fibrosis by reducing mitochondrial (mt) DNA damage and pro-fibrotic monocyte recruitment-both are implicated in the pathobiology of pulmonary fibrosis. Bleomycin (1.5 U/kg), crocidolite asbestos (100 µg/50 µL) or controls was intratracheally instilled in Wild-Type ( C57Bl6) mice. PF543 (1 mg/kg) or vehicle was intraperitoneally injected once every two days from day 7-21 following bleomycin and day 14-21 or day 30-60 following asbestos. PF543 reduced bleomycin- and asbestos-induced pulmonary fibrosis at both time points as well as lung expression of profibrotic markers, lung mtDNA damage, and fibrogenic monocyte recruitment. In contrast to human lung fibroblasts, asbestos augmented lung epithelial cell (MLE) mtDNA damage and PF543 was protective. Post-exposure PF543 mitigates pulmonary fibrosis in part by reducing lung epithelial cell mtDNA damage and monocyte recruitment. We reason that SPHK1 signaling may be an innovative therapeutic target for managing patients with IPF and other forms of lung fibrosis.
Databáze:	MEDLINE
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