Whole Genome Sequencing of Peruvian Klebsiella pneumoniae Identifies Novel Plasmid Vectors Bearing Carbapenem Resistance Gene NDM-1 .

Autor: Roach D; University of Washington School of Medicine, Department of Internal Medicine, Seattle, Washington, USA.; University of Washington School of Medicine, Department of Global Health, Seattle, Washington, USA., Waalkes A; University of Washington School of Medicine, Department of Laboratory Medicine, Seattle, Washington, USA., Abanto J; Hospital Nacional Dos de Mayo, Lima, Peru., Zunt J; University of Washington School of Medicine, Department of Internal Medicine, Seattle, Washington, USA.; University of Washington School of Medicine, Department of Global Health, Seattle, Washington, USA., Cucho C; Hospital Nacional Dos de Mayo, Lima, Peru., Soria J; Hospital Nacional Dos de Mayo, Lima, Peru., Salipante SJ; University of Washington School of Medicine, Department of Laboratory Medicine, Seattle, Washington, USA.
Jazyk: angličtina
Zdroj: Open forum infectious diseases [Open Forum Infect Dis] 2020 Jul 02; Vol. 7 (8), pp. ofaa266. Date of Electronic Publication: 2020 Jul 02 (Print Publication: 2020).
DOI: 10.1093/ofid/ofaa266
Abstrakt: Background: Klebsiella pneumoniae is a bacterial pathogen with increasing rates of resistance to carbapenem antibiotics, but the population structure and genetic drivers of carbapenem-resistant K pneumoniae (CRKP) remain underexplored in developing countries. Carbapenem-resistant K pneumoniae were recently introduced into Peru but have grown rapidly in prevalence, enabling study of this pathogen as it expands into an unaffected environment.
Methods: In this study, using whole genome sequencing, we show that 3 distinct lineages encompass almost all CRKP identified in the hospital where it was first reported in Peru.
Results: The most prevalent lineage, ST348, has not been described outside of Europe, raising concern for global dissemination. We identified metallo- β -lactamase NDM-1 as the primary carbapenem resistance effector, which was harbored on a novel vector resulting from recombination between 2 different plasmids, pKP1-NDM-1 and pMS7884A.
Conclusions: This study is the first of its kind performed in Peru, and it furthers our understanding of the landscape of CRKP infections in Latin America.
(© The Author(s) 2020. Published by Oxford University Press on behalf of Infectious Diseases Society of America.)
Databáze: MEDLINE
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