ddRAD-seq derived genome-wide SNPs, high density linkage map and QTLs for fruit quality traits in strawberry ( Fragaria x ananassa ).

Autor: Natarajan S; Department of Horticulture, Suncheon National University, 255 Jungang-ro, Suncheon, Jeonnam 57922 Republic of Korea., Hossain MR; Department of Horticulture, Suncheon National University, 255 Jungang-ro, Suncheon, Jeonnam 57922 Republic of Korea., Kim HT; Department of Horticulture, Suncheon National University, 255 Jungang-ro, Suncheon, Jeonnam 57922 Republic of Korea., Denison MIJ; Department of Horticulture, Suncheon National University, 255 Jungang-ro, Suncheon, Jeonnam 57922 Republic of Korea., Ferdous MJ; Department of Horticulture, Suncheon National University, 255 Jungang-ro, Suncheon, Jeonnam 57922 Republic of Korea., Jung HJ; Department of Horticulture, Suncheon National University, 255 Jungang-ro, Suncheon, Jeonnam 57922 Republic of Korea., Park JI; Department of Horticulture, Suncheon National University, 255 Jungang-ro, Suncheon, Jeonnam 57922 Republic of Korea., Nou IS; Department of Horticulture, Suncheon National University, 255 Jungang-ro, Suncheon, Jeonnam 57922 Republic of Korea.
Jazyk: angličtina
Zdroj: 3 Biotech [3 Biotech] 2020 Aug; Vol. 10 (8), pp. 353. Date of Electronic Publication: 2020 Jul 27.
DOI: 10.1007/s13205-020-02291-5
Abstrakt: Understanding the genetic determinants are essential for improving the fruit quality traits of strawberry. In this study, we focused on mapping the loci for fruit-length (FL), -diameter (FD), -weight (FW) and -soluble solid content (SSC) using the genome-wide single nucleotide polymorphisms (SNPs) identified via ddRAD-sequencing of the F 1 population raised from Maehyang (♀) X Festival (♂). A total of 12,698 high quality SNPs were identified of which 1554 SNPs that showed significant Mendelian segregation ( p  < 0.05) were mapped to 53 linkage groups (LG) spanning a total of 2937.93 cM with an average marker density of 2.14 cM/locus. Six QTLs for FL and four QTLs for each of FD, FW and SSC were identified that explained 24-35%, 21-42%, 24-54% and 23-50% of overall phenotypic variations, respectively. The genes that lie within these QTL regions were extracted and discussed thoroughly. In addition, a high resolution melting marker (MF154) were designed based on the SNP A1 723 G of the UDP-glucose 4-epimerase GEPI48-like gene FAN_iscf00021287. The marker detected the high vs low sugar containing F 1 plants and commercial cultivars with 81.39% and 86.95% detection accuracy, respectively. These SNPs, linkage map, QTLs and candidate genes will be helpful in understanding and improving the fruit quality traits of strawberry.
Competing Interests: Conflict of interestThe authors declare that they have no competing interests.
(© The Author(s) 2020.)
Databáze: MEDLINE
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