Bacteria from tropical semiarid temporary ponds promote maize growth under hydric stress.

Autor: Prudêncio de Araújo VLV; Universidade Federal Rural de Pernambuco, Departamento de Agronomia, Recife, Pernambuco, 52171-900, Brazil. Electronic address: victorlucas395@hotmail.com., Lira Junior MA; Universidade Federal Rural de Pernambuco, Departamento de Agronomia, Recife, Pernambuco, 52171-900, Brazil. Electronic address: mario.alirajr@ufrpe.br., Souza Júnior VS; Universidade Federal Rural de Pernambuco, Departamento de Agronomia, Recife, Pernambuco, 52171-900, Brazil. Electronic address: valdomiro.souzajunior@ufrpe.br., de Araújo Filho JC; Empresa Brasileira de Pesquisa Agropecuária (Embrapa Solos), Unidade de Execução de Pesquisa (UEP), Recife, Pernambuco, 51020-240, Brazil. Electronic address: jose.coelho@embrapa.br., Cury Fracetto FJ; Universidade Federal Rural de Pernambuco, Departamento de Agronomia, Recife, Pernambuco, 52171-900, Brazil. Electronic address: curyfelipe@hotmail.com., Andreote FD; Universidade de São Paulo, Escola Superior de Agricultura 'Luiz de Queiroz', Departamento de Ciência do Solo, Piracicaba, São Paulo, 13400-970, Brazil. Electronic address: fdandreo@usp.br., de Araujo Pereira AP; Universidade Federal do Ceará, Centro de Ciências Agrárias, Departamento de Ciências do Solo, Fortaleza, Ceará, 60355-636, Brazil. Electronic address: arthur.prudencio@usp.br., Mendes Júnior JP; Universidade Federal Rural de Pernambuco, Departamento de Agronomia, Recife, Pernambuco, 52171-900, Brazil. Electronic address: jpetroniomendes@hotmail.com., Rêgo Barros FMD; Universidade de São Paulo, Escola Superior de Agricultura 'Luiz de Queiroz', Departamento de Ciência do Solo, Piracicaba, São Paulo, 13400-970, Brazil. Electronic address: felipemartins.martins642@gmail.com., Monteiro Fracetto GG; Universidade Federal Rural de Pernambuco, Departamento de Agronomia, Recife, Pernambuco, 52171-900, Brazil. Electronic address: giselle.fracetto@ufrpe.br.
Jazyk: angličtina
Zdroj: Microbiological research [Microbiol Res] 2020 Nov; Vol. 240, pp. 126564. Date of Electronic Publication: 2020 Jul 31.
DOI: 10.1016/j.micres.2020.126564
Abstrakt: World climate change has triggered soil water stress and imposed limitations on agricultural production. Plant growth-promoting bacteria (PGPBs) have been an efficient strategy to improve the biological supply and growth of plants under distinct abiotic stress conditions. We hypothesized that the soils from a temporary pond may harbor PGPBs with potential strains which increase maize tolerance to water deficit. We studied rhizosphere and bulk soil of Mimosa bimucronata in a temporary pond from semiarid Northeast Brazil to access strains with characteristics to promote plant growth and mitigate abiotic stress for maize crop. We isolated 355 bacterial isolates, from which 96 were selected based on the morphophysiological characterization to assess IAA production (42 % produced over 50 μg mL -1 of IAA), calcium phosphate solubilization (with one isolate achieving medium IS), biofilm and exopolysaccharides production (66 % and 98 % of isolates, respectively). Based on these mechanisms, the 30 most promising bacterial isolates were selected to assess biological nitrogen fixation (74 % of the isolates showed nitrogenase activity greater than 20 C 2 H 4 .h -1 .mg -1 ), ACC deaminase activity (80 % of isolates) and growth in medium with reduced water activity (8 % of isolates grew in medium with water activity (A w ) of 0.844). We sequenced the 16S rRNA gene from the seven most promising isolates in in vitro and in vivo assays, which were identified as Staphylococcus edaphicus, Bacillus wiedmannii, Micrococcus yunnanensis, Streptomyces alboflavus, Streptomyces alboflavus, Bacillus wiedmanni and Bacillus cereus. In vivo, eleven isolates and three bacterial consortia did not differ from the control with nutrient solution, for total leaf area and root dry mass of maize. S. alboflavus (BS43) had the best in vivo results, not differing from the control with nutrient solution. We highlight the unpublished potential of Staphylococcus edaphicus and Streptomyces alboflavus in promoting the growth of plants under water stress. In addition, it is the first report of bacteria isolated from a temporary pond in the Brazilian semiarid which promoting plant growth attributes and development.
(Copyright © 2020 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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