Evaluation of the new Id-Fungi plates from Conidia for MALDI-TOF MS identification of filamentous fungi and comparison with conventional methods as identification tool for dermatophytes from nails, hair and skin samples.

Autor: Sacheli R; Department of Clinical Microbiology, National Reference Center for Mycosis, Center for Interdisciplinary research on Medicines (CIRM), University Hospital of Liege, Liege, Belgium., Henri AS; Department of Clinical Microbiology, National Reference Center for Mycosis, Center for Interdisciplinary research on Medicines (CIRM), University Hospital of Liege, Liege, Belgium., Seidel L; Department of Biostatistics, University Hospital of Liege, Liege, Belgium., Ernst M; Department of Biostatistics, University Hospital of Liege, Liege, Belgium., Darfouf R; Department of Clinical Microbiology, National Reference Center for Mycosis, Center for Interdisciplinary research on Medicines (CIRM), University Hospital of Liege, Liege, Belgium., Adjetey C; Department of Clinical Microbiology, National Reference Center for Mycosis, Center for Interdisciplinary research on Medicines (CIRM), University Hospital of Liege, Liege, Belgium., Schyns M; Department of Clinical Microbiology, National Reference Center for Mycosis, Center for Interdisciplinary research on Medicines (CIRM), University Hospital of Liege, Liege, Belgium., Marechal L; Department of Dermatopathology, University Hospital of Liege, Liege, Belgium., Meex C; Department of Clinical Microbiology, National Reference Center for Mycosis, Center for Interdisciplinary research on Medicines (CIRM), University Hospital of Liege, Liege, Belgium., Arrese J; Department of Dermatopathology, University Hospital of Liege, Liege, Belgium., Hayette MP; Department of Clinical Microbiology, National Reference Center for Mycosis, Center for Interdisciplinary research on Medicines (CIRM), University Hospital of Liege, Liege, Belgium.
Jazyk: angličtina
Zdroj: Mycoses [Mycoses] 2020 Oct; Vol. 63 (10), pp. 1115-1127. Date of Electronic Publication: 2020 Sep 04.
DOI: 10.1111/myc.13156
Abstrakt: Objectives: We first compare the efficiency of mould/dermatophyte identification by MALDI-TOF MS using a new medium called Id-Fungi plates (IDFP) from Conidia ® and two different databases. For the second purpose, we evaluated a new version of the medium supplemented with cycloheximide, Id-Fungi plates Plus (IDFPC) for the direct inoculation of nails, hair and skin samples and compared the efficiency of MALDI-TOF MS identification of dermatophytes to classical methods based on culture and microscopy.
Methods: A total of 71 strains have been cultured IDFP and Sabouraud gentamicin plates (SGC2) and were identified by MALDI-TOF MS. For the evaluation of the combination IDFPC/ MALDI-TOF MS as a method of identification for dermatophytes, 428 samples of hair nails and skin were cultivated in parallel on IDFPC and Sabouraud + cycloheximide medium (SAB-ACTI).
Results: For Aspergillus sp and non-Aspergillus moulds, the best performances were obtained on IDFP after maximum 48-h growth, following protein extraction. For dermatophytes, the best condition was using the IDFP at 72 hours, after extended direct deposit. Regarding the direct inoculation of nails, hair skin on IDFPC, 129/428 (30.1%) showed a positive culture against 150/428 (35%) on SAB-ACTI medium. Among the 129 positive strains, the identification by MALDI-TOF MS was correct for 92/129 (71.4%).
Conclusion: The IDFP allows the generation of better spectra by MALDI-TOF MS compared to SGC2. It facilitates sampling and deposit. Regarding the use of IDFPC, this medium seems less sensitive than SAB-ACTI but among positive strains, the rate of correct identification by MALDI-TOF MS is satisfactory.
(© 2020 Wiley-VCH GmbH.)
Databáze: MEDLINE
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