Frequency of congenital cytomegalovirus infections in newborns in the Sao Paulo State, 2010-2018.

Autor: Figueiredo CG; Centro de Virologia, Instituto Adolfo Lutz, São Paulo, São Paulo, Brazil., Luchs A; Centro de Virologia, Instituto Adolfo Lutz, São Paulo, São Paulo, Brazil., Durigon EL; Instituto de Ciências Biomédicas II, Universidade de São Paulo, São Paulo, São Paulo, Brazil., Oliveira DBL; Instituto de Ciências Biomédicas II, Universidade de São Paulo, São Paulo, São Paulo, Brazil., Silva VBD; Instituto de Ciências Biomédicas II, Universidade de São Paulo, São Paulo, São Paulo, Brazil., Mello RM; Instituto de Ciências Biomédicas II, Universidade de São Paulo, São Paulo, São Paulo, Brazil., Afonso AMS; Centro de Virologia, Instituto Adolfo Lutz, São Paulo, São Paulo, Brazil., Oliveira MI; Centro de Virologia, Instituto Adolfo Lutz, São Paulo, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Revista do Instituto de Medicina Tropical de Sao Paulo [Rev Inst Med Trop Sao Paulo] 2020; Vol. 62, pp. e54. Date of Electronic Publication: 2020 Aug 03.
DOI: 10.1590/s1678-9946202062054
Abstrakt: Human cytomegalovirus (HCMV) infections remain a neglected public health issue. The aim of the present study was to evaluate the frequency of HCMV congenital infections in newborns up to 1 month in the Sao Paulo State, from 2010 to 2018. The molecular characterization of HCMV-positive samples was also undertaken. Urine samples from 275 potential congenital HCMV-infected patients were tested by real-time Polymerase Chain Reaction (qPCR). HCMV-positive samples were amplified by conventional PCR targeting the UL89 gene, sequenced and searched for mutations. A total of 32 (11.6%) positive-HCMV cases were detected (mean Ct 30.59); mean and median age of 10.3 and 6 days old, respectively. Children aged between 0-3 weeks had higher HCMV detection rates (84.4%; 27/32). UL89 gene was successfully sequenced in two samples, both classified as the human betaherpesvirus 5. No described resistance-associated mutations were identified. A routine screening in newborns coupled with the genetic characterization of key viral genes is vital to decrease sequels associated with congenital HCMV infections.
Databáze: MEDLINE
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