Procyanidin A2, a polyphenolic compound, exerts anti-inflammatory and anti-oxidative activity in lipopolysaccharide-stimulated RAW264.7 cells.

Autor: Wang QQ; College of Pharmacy, Guangxi University of Chinese Medicine, Nanning, China.; Guangxi Engineering Technology Research Center of Advantage Chinese Patent Drug and Ethnic Drug Development, Nanning, China., Gao H; College of Pharmacy, Guangxi University of Chinese Medicine, Nanning, China.; Guangxi Engineering Technology Research Center of Advantage Chinese Patent Drug and Ethnic Drug Development, Nanning, China., Yuan R; College of Pharmacy, Guangxi University of Chinese Medicine, Nanning, China., Han S; College of Pharmacy, Guangxi University of Chinese Medicine, Nanning, China.; Guangxi Engineering Technology Research Center of Advantage Chinese Patent Drug and Ethnic Drug Development, Nanning, China., Li XX; College of Pharmacy, Guangxi University of Chinese Medicine, Nanning, China.; Guangxi Engineering Technology Research Center of Advantage Chinese Patent Drug and Ethnic Drug Development, Nanning, China., Tang M; College of Pharmacy, Guangxi University of Chinese Medicine, Nanning, China., Dong B; College of Public Health and Management, Guangxi University of Chinese Medicine, Nanning, China., Li JX; College of Pharmacy, Guangxi University of Chinese Medicine, Nanning, China.; Guangxi Engineering Technology Research Center of Advantage Chinese Patent Drug and Ethnic Drug Development, Nanning, China., Zhao LC; College of Pharmacy, Guangxi University of Chinese Medicine, Nanning, China.; Guangxi Engineering Technology Research Center of Advantage Chinese Patent Drug and Ethnic Drug Development, Nanning, China., Feng J; College of Pharmacy, Guangxi University of Chinese Medicine, Nanning, China.; Guangxi Engineering Technology Research Center of Advantage Chinese Patent Drug and Ethnic Drug Development, Nanning, China., Yang S; College of Pharmacy, Guangxi University of Chinese Medicine, Nanning, China.; Guangxi Engineering Technology Research Center of Advantage Chinese Patent Drug and Ethnic Drug Development, Nanning, China.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2020 Aug 05; Vol. 15 (8), pp. e0237017. Date of Electronic Publication: 2020 Aug 05 (Print Publication: 2020).
DOI: 10.1371/journal.pone.0237017
Abstrakt: Procyandin A2 (PCA2) is a polyphenolic compound which is isolated from grape seeds. It has been reported that PCA2 exhibits antioxidative and anti-inflammatory effects, but its molecular mechanism is still poorly understood. This study tests the hypothesis that PCA2 suppresses lipopolysaccharide (LPS)-induced inflammation and oxidative stress through targeting the nuclear factor-κB (NF-κB), mitogen-activated protein kinase (MAPK), and NF-E2-related factor 2 (Nrf2) pathways in RAW264.7 cells. PCA2 (20, 40, 80 μM) exhibited no significant cytotoxicity in RAW264.7 cells and showed an inhibitory effect on an LPS-induced nitrite level. Pro-inflammatory cytokines like tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), prostaglandin E2 (PGE2), nitric oxide (NO), and reactive oxygen species (ROS) were suppressed by PCA2 with a concentration range of 0-80 μM. The mRNA levels of TNF-α and IL-6 were inhibited by PCA2 (80 μM). The hallmark-protein expression of the NF-κB (p-IKKα/β, p-IκBα, and p-p65) and MAPK (p-p38, p-JNK, and p-ERK) pathways were decreased by PCA2 in LPS-stimulated RAW264.7 cells. In addition, immunofluorescence results indicated that PCA2 (80 μM) promoted the translocation of NF-κB/p65 from the cytoplasm into the nucleus. PCA2 upregulated the expressions of Nrf2 and HO-1 and downregulated the expression of Keap-1. Simultaneously, PCA2 (80 μM) reversed LPS-induced Nrf2 translocation from the nucleus into the cytoplasm. Collectively, PCA2 protect cells against the damage from inflammation and oxidative injury, which suggest a potential therapeutic strategy for inflammatory and oxidative stress through targeting NF-κB, MAPK, and Nrf2 pathways in RAW264.7 cells.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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