| Autor: |
Moragas-Tellis CJ; Natural Products Department, Pharmaceutical Technology Institute-Farmanguinhos, Rio de Janeiro, Rio de Janeiro 21040-900, Brazil., Almeida-Souza F; Laboratório de Imunomodulação e Protozoologia, Instituto Oswaldo Cruz, Oswaldo Cruz Foundation, Rio de Janeiro 21040-360, Brazil.; Postgraduate in Animal Science, Universidade Estadual do Maranhão, São Luís 65055-310, Maranhão, Brazil., Chagas MDSDS; Natural Products Department, Pharmaceutical Technology Institute-Farmanguinhos, Rio de Janeiro, Rio de Janeiro 21040-900, Brazil., Souza PVR; Natural Products Department, Pharmaceutical Technology Institute-Farmanguinhos, Rio de Janeiro, Rio de Janeiro 21040-900, Brazil., Silva-Silva JV; Laboratório de Imunomodulação e Protozoologia, Instituto Oswaldo Cruz, Oswaldo Cruz Foundation, Rio de Janeiro 21040-360, Brazil., Ramos YJ; Natural Products Department, Pharmaceutical Technology Institute-Farmanguinhos, Rio de Janeiro, Rio de Janeiro 21040-900, Brazil., Moreira DL; Natural Products Department, Pharmaceutical Technology Institute-Farmanguinhos, Rio de Janeiro, Rio de Janeiro 21040-900, Brazil., Calabrese KDS; Laboratório de Imunomodulação e Protozoologia, Instituto Oswaldo Cruz, Oswaldo Cruz Foundation, Rio de Janeiro 21040-360, Brazil., Behrens MD; Natural Products Department, Pharmaceutical Technology Institute-Farmanguinhos, Rio de Janeiro, Rio de Janeiro 21040-900, Brazil. |
| Abstrakt: |
Arrabidaea chica Verlot (crajiru) is a plant used in folk medicine as an astringent, anti-inflammatory, wound healing and to treat fungal and viral diseases such as measles chickenpox and herpes. Arrabidaea chica has several morphotypes recognized but little is known about its chemical variability. In the present study the anthocyanidin profile of A. chica morphotypes collected in two seasons (summer and winter) have been examined and their activity against Leishmania infection compared. High-performance liquid chromatography coupled to a diode-array detector (HPLC-DAD-UV) and by tandem mass spectrometry with electrospray ionization (ESI-MS/MS) were used for anthocyanidin separation and identification. Antileishmanial activity was measured against promastigote forms of Leishmania amazonensis . Multivariate analysis, principal component analysis (PCA) and Pearson's correlation were performed to classify morphotypes accordingly to their anthocyanidin profile. The presence of 6,7,3',4'-tetrahydroxy-5-methoxyflavylium (3'-hydroxy-carajurone) (1), carajurone (2), 6,7,3'-trihydroxy-5,4'-dimethoxy-flavylium (3'-hydroxy-carajurin) (3) and carajurin (4), and three unidentified anthocyanidins were detected. Two different groups were recognized: group I containing 3'-hydroxy-carajurone; and group II with high content of carajurin. Among anthocyanidins identified in the extracts, only carajurin showed significant statistical correlation ( p = 0.030) with activity against L. amazonensis . Carajurin could thus be considered as a pharmacological marker for the antileishmanial potential of the species. |
