Linking metabolic phenotypes to pathogenic traits among "Candidatus Liberibacter asiaticus" and its hosts.

Autor: Zuñiga C; Department of Pediatrics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093-0760, USA., Peacock B; Department of Microbiology and Plant Pathology, University of California, Riverside, 900 University Avenue, Riverside, CA, 92521, USA., Liang B; Department of Pediatrics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093-0760, USA.; State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai, People's Republic of China., McCollum G; USDA, ARS, US Horticultural Research Laboratory, 2001 S. Rock Road, Fort Pierce, FL, 34945, USA., Irigoyen SC; Texas A&M AgriLife Research and Extension Center, Texas A&M University System, Weslaco, TX, USA., Tec-Campos D; Department of Pediatrics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093-0760, USA.; Facultad de Ingeniería Química, Universidad Autónoma de Yucatán, Campus de Ciencias Exactas e Ingenierías, Mérida, 97203, Yucatán, México., Marotz C; Department of Pediatrics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093-0760, USA., Weng NC; Department of Pediatrics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093-0760, USA., Zepeda A; Facultad de Ingeniería Química, Universidad Autónoma de Yucatán, Campus de Ciencias Exactas e Ingenierías, Mérida, 97203, Yucatán, México., Vidalakis G; Department of Microbiology and Plant Pathology, University of California, Riverside, 900 University Avenue, Riverside, CA, 92521, USA., Mandadi KK; Texas A&M AgriLife Research and Extension Center, Texas A&M University System, Weslaco, TX, USA.; Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX, USA., Borneman J; Department of Microbiology and Plant Pathology, University of California, Riverside, 900 University Avenue, Riverside, CA, 92521, USA. borneman@ucr.edu., Zengler K; Department of Pediatrics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093-0760, USA. kzengler@ucsd.edu.; Department of Bioengineering, University of California, San Diego, La Jolla, CA, 92093-0412, USA. kzengler@ucsd.edu.; Center for Microbiome Innovation, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093-0403, USA. kzengler@ucsd.edu.
Jazyk: angličtina
Zdroj: NPJ systems biology and applications [NPJ Syst Biol Appl] 2020 Aug 04; Vol. 6 (1), pp. 24. Date of Electronic Publication: 2020 Aug 04.
DOI: 10.1038/s41540-020-00142-w
Abstrakt: Candidatus Liberibacter asiaticus (CLas) has been associated with Huanglongbing, a lethal vector-borne disease affecting citrus crops worldwide. While comparative genomics has provided preliminary insights into the metabolic capabilities of this uncultured microorganism, a comprehensive functional characterization is currently lacking. Here, we reconstructed and manually curated genome-scale metabolic models for the six CLas strains A4, FL17, gxpsy, Ishi-1, psy62, and YCPsy, in addition to a model of the closest related culturable microorganism, L. crescens BT-1. Predictions about nutrient requirements and changes in growth phenotypes of CLas were confirmed using in vitro hairy root-based assays, while the L. crescens BT-1 model was validated using cultivation assays. Host-dependent metabolic phenotypes were revealed using expression data obtained from CLas-infected citrus trees and from the CLas-harboring psyllid Diaphorina citri Kuwayama. These results identified conserved and unique metabolic traits, as well as strain-specific interactions between CLas and its hosts, laying the foundation for the development of model-driven Huanglongbing management strategies.
Databáze: MEDLINE
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