| Autor: |
Palazzo M; Department of Agricultural, Environmental and Food Sciences, University of Molise, 86100 Campobasso, Italy., Vizzarri F; Department of Agricultural and Environmental Sciences, University of Bari Aldo Moro, 70126 Bari, Italy., Ondruška L; National Agricultural and Food Centre, 949 01 Nitra, Slovakia., Rinaldi M; Clinica Oculistica, Unicampania, 80131 Napoli, Italy., Pacente L; Corneal Transplant Center, Pellegrini Hospital, 80134 Naples, Italy., Guerra G; Department of Medicine and Health Science 'V. Tiberio', University of Molise, 86100 Campobasso, Italy., Merolla F; Department of Medicine and Health Science 'V. Tiberio', University of Molise, 86100 Campobasso, Italy., Caruso C; Corneal Transplant Center, Pellegrini Hospital, 80134 Naples, Italy., Costagliola C; Department of Medicine and Health Science 'V. Tiberio', University of Molise, 86100 Campobasso, Italy. |
| Abstrakt: |
This study aimed to evaluate the protective effect of a topical antioxidant and ultraviolet (UV) shielding action formulation containing riboflavin and D-α-tocopherol polyethylene glycol succinate (TPGS) vitamin E against corneal UV-induced damage in vivo rabbit eyes. In vivo experiments were performed using male albino rabbits, which were divided into four groups. The control group (CG) did not receive any UV irradiation; the first group (IG) was irradiated with a UV-B-UV-A lamp for 30 min; the second (G30) and third (G60) groups received UV irradiation for 30 and 60 min, respectively, and were topically treated with one drop of the antioxidant and shielding formulation every 15 min, starting one hour before irradiation, until the end of UV exposure. The cornea of the IG group showed irregular thickening, detachment of residual fragments of the Descemet membrane, stromal fluid swelling with consequent collagen fiber disorganization and disruption, and inflammation. The cornea of the G30 group showed edema, a mild thickening of the Descemet membrane without fibrillar collagen disruption and focal discoloration, or inflammation. In the G60 group, the cornea showed a more severe thickening, a more abundant fluid accumulation underneath the Descemet membrane with focal detachment, and no signs of severe tissue alterations, as were recorded in the IG group. Our results demonstrate that topical application of eye drops containing riboflavin and TPGS vitamin E counteracts UV corneal injury in exposed rabbits. |
