Development and validation of a universal blood donor genotyping platform: a multinational prospective study.

Autor: Gleadall NS; Department of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.; NHS Blood and Transplant, Cambridge Biomedical Campus, Cambridge, United Kingdom., Veldhuisen B; Department of Experimental Immunohematology, Sanquin Research and Landsteiner Laboratory, Amsterdam University Medical Center (AUMC), Amsterdam, The Netherlands.; Department of Diagnostic Immunohematology, Sanquin Diagnostic Services, Amsterdam, The Netherlands., Gollub J; Thermo Fisher Scientific, Santa Clara, CA., Butterworth AS; British Heart Foundation Cardiovascular Epidemiology Unit, Department of Public Health and Primary Care, University of Cambridge, Cambridge, United Kingdom.; National Institute for Health Research Blood and Transplant Research Unit in Donor Health and Genomics, University of Cambridge, Cambridge, United Kingdom.; Health Data Research UK Cambridge, Wellcome Genome Campus and University of Cambridge, Cambridge, United Kingdom., Ord J; Department of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.; NHS Blood and Transplant, Cambridge Biomedical Campus, Cambridge, United Kingdom., Penkett CJ; Department of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.; NIHR BioResource, Cambridge University Hospitals, Cambridge Biomedical Campus, Cambridge, United Kingdom., Timmer TC; Department of Donor Medicine Research-Donor Studies, Sanquin Research, Amsterdam, The Netherlands.; Department of Public Health and Landsteiner Laboratory, AUMC, University of Amsterdam, Amsterdam, The Netherlands., Sauer CM; Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom., van der Bolt N; Department of Experimental Immunohematology, Sanquin Research and Landsteiner Laboratory, Amsterdam University Medical Center (AUMC), Amsterdam, The Netherlands., Brown C; Histocompatibility and Immunogenetics Laboratory, NHS Blood and Transplant-Colindale Centre, London, United Kingdom., Brugger K; Computational Biology Unit, Department of Informatics, University of Bergen, Bergen, Norway., Dilthey AT; Institute of Medical Microbiology, University Hospital of Dusseldorf, Dusseldorf, Germany.; Genome Informatics Section, Computational and Statistical Genomics Branch, National Human Genome Research Institute, Bethesda, MD., Duarte D; Department of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.; NIHR BioResource, Cambridge University Hospitals, Cambridge Biomedical Campus, Cambridge, United Kingdom., Grimsley S; International Blood Group Reference Laboratory, NHS Blood and Transplant, Bristol, United Kingdom., van den Hurk K; Department of Donor Medicine Research-Donor Studies, Sanquin Research, Amsterdam, The Netherlands., Jongerius JM; National Screening Laboratory, Sanquin Research and Laboratory Services, Sanquin Research, Amsterdam, The Netherlands., Luken J; Department of Diagnostic Immunohematology, Sanquin Diagnostic Services, Amsterdam, The Netherlands., Megy K; Department of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.; NIHR BioResource, Cambridge University Hospitals, Cambridge Biomedical Campus, Cambridge, United Kingdom., Miflin G; NHS Blood and Transplant, London, United Kingdom., Nelson CS; Thermo Fisher Scientific, Santa Clara, CA., Prinsze FJ; Department of Donor Medicine Research-Donor Studies, Sanquin Research, Amsterdam, The Netherlands., Sambrook J; Department of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.; NIHR BioResource, Cambridge University Hospitals, Cambridge Biomedical Campus, Cambridge, United Kingdom., Simeoni I; Department of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.; NIHR BioResource, Cambridge University Hospitals, Cambridge Biomedical Campus, Cambridge, United Kingdom., Sweeting M; British Heart Foundation Cardiovascular Epidemiology Unit, Department of Public Health and Primary Care, University of Cambridge, Cambridge, United Kingdom.; National Institute for Health Research Blood and Transplant Research Unit in Donor Health and Genomics, University of Cambridge, Cambridge, United Kingdom.; Department of Health Sciences, University of Leicester, Leicester, United Kingdom., Thornton N; International Blood Group Reference Laboratory, NHS Blood and Transplant, Bristol, United Kingdom., Trompeter S; NHS Blood and Transplant, London, United Kingdom.; University College London NHS Foundation Trust, London, United Kingdom., Tuna S; Department of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.; NIHR BioResource, Cambridge University Hospitals, Cambridge Biomedical Campus, Cambridge, United Kingdom., Varma R; Thermo Fisher Scientific, Santa Clara, CA., Walker MR; British Heart Foundation Cardiovascular Epidemiology Unit, Department of Public Health and Primary Care, University of Cambridge, Cambridge, United Kingdom.; National Institute for Health Research Blood and Transplant Research Unit in Donor Health and Genomics, University of Cambridge, Cambridge, United Kingdom., Danesh J; British Heart Foundation Cardiovascular Epidemiology Unit, Department of Public Health and Primary Care, University of Cambridge, Cambridge, United Kingdom.; National Institute for Health Research Blood and Transplant Research Unit in Donor Health and Genomics, University of Cambridge, Cambridge, United Kingdom.; Health Data Research UK Cambridge, Wellcome Genome Campus and University of Cambridge, Cambridge, United Kingdom.; Department of Human Genetics, Wellcome Sanger Institute, Hinxton, United Kingdom., Roberts DJ; National Institute for Health Research Blood and Transplant Research Unit in Donor Health and Genomics, University of Cambridge, Cambridge, United Kingdom.; NHS Blood and Transplant, Oxford, United Kingdom.; NIHR Oxford Biomedical Research Centre-Haematology Theme and Radcliffe Department of Medicine, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom., Ouwehand WH; Department of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.; NHS Blood and Transplant, Cambridge Biomedical Campus, Cambridge, United Kingdom.; National Institute for Health Research Blood and Transplant Research Unit in Donor Health and Genomics, University of Cambridge, Cambridge, United Kingdom.; Department of Human Genetics, Wellcome Sanger Institute, Hinxton, United Kingdom., Stirrups KE; Department of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.; NIHR BioResource, Cambridge University Hospitals, Cambridge Biomedical Campus, Cambridge, United Kingdom., Rendon A; Department of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom.; Genomics England, London, United Kingdom., Westhoff CM; Immunohematology and Genomics, New York Blood Center, New York., Di Angelantonio E; British Heart Foundation Cardiovascular Epidemiology Unit, Department of Public Health and Primary Care, University of Cambridge, Cambridge, United Kingdom.; National Institute for Health Research Blood and Transplant Research Unit in Donor Health and Genomics, University of Cambridge, Cambridge, United Kingdom.; Health Data Research UK Cambridge, Wellcome Genome Campus and University of Cambridge, Cambridge, United Kingdom., van der Schoot CE; Department of Experimental Immunohematology, Sanquin Research and Landsteiner Laboratory, Amsterdam University Medical Center (AUMC), Amsterdam, The Netherlands., Astle WJ; NHS Blood and Transplant, Cambridge Biomedical Campus, Cambridge, United Kingdom.; National Institute for Health Research Blood and Transplant Research Unit in Donor Health and Genomics, University of Cambridge, Cambridge, United Kingdom.; MRC Biostatistics Unit, Cambridge Biomedical Campus, Cambridge, United Kingdom., Watkins NA; NHS Blood and Transplant, Cambridge Biomedical Campus, Cambridge, United Kingdom., Lane WJ; Department of Pathology, Brigham and Women's Hospital, Boston, MA; and.; Harvard Medical School, Boston, MA.
Jazyk: angličtina
Zdroj: Blood advances [Blood Adv] 2020 Aug 11; Vol. 4 (15), pp. 3495-3506.
DOI: 10.1182/bloodadvances.2020001894
Abstrakt: Each year, blood transfusions save millions of lives. However, under current blood-matching practices, sensitization to non-self-antigens is an unavoidable adverse side effect of transfusion. We describe a universal donor typing platform that could be adopted by blood services worldwide to facilitate a universal extended blood-matching policy and reduce sensitization rates. This DNA-based test is capable of simultaneously typing most clinically relevant red blood cell (RBC), human platelet (HPA), and human leukocyte (HLA) antigens. Validation was performed, using samples from 7927 European, 27 South Asian, 21 East Asian, and 9 African blood donors enrolled in 2 national biobanks. We illustrated the usefulness of the platform by analyzing antibody data from patients sensitized with multiple RBC alloantibodies. Genotyping results demonstrated concordance of 99.91%, 99.97%, and 99.03% with RBC, HPA, and HLA clinically validated typing results in 89 371, 3016, and 9289 comparisons, respectively. Genotyping increased the total number of antigen typing results available from 110 980 to >1 200 000. Dense donor typing allowed identification of 2 to 6 times more compatible donors to serve 3146 patients with multiple RBC alloantibodies, providing at least 1 match for 176 individuals for whom previously no blood could be found among the same donors. This genotyping technology is already being used to type thousands of donors taking part in national genotyping studies. Extraction of dense antigen-typing data from these cohorts provides blood supply organizations with the opportunity to implement a policy of genomics-based precision matching of blood.
Databáze: MEDLINE