Transforming growth factor-β boosts the functionality of human bone marrow-derived mesenchymal stromal cells.

Autor: Kale VP; Symbiosis Centre for Stem Cell Research, Symbiosis International University, Pune, India.
Jazyk: angličtina
Zdroj: Cell biology international [Cell Biol Int] 2020 Nov; Vol. 44 (11), pp. 2293-2306. Date of Electronic Publication: 2020 Aug 12.
DOI: 10.1002/cbin.11437
Abstrakt: Transforming growth factor β1 (TGFβ1) is a negative regulator of hematopoiesis, and yet, it is frequently found at the active sites of hematopoiesis. Here, we show for the first time that bone marrow-derived mononuclear cells (BM MNCs) secrete TGFβ1 in response to erythropoietin (EPO). We further show that human bone marrow-derived mesenchymal stromal cells (BMSCs) briefly exposed to the conditioned medium of EPO-primed MNCs, or purified TGFβ1, gain significantly increased hematopoiesis-supportive ability. Mechanistically, we show that this phenomenon involves TGFβ1-mediated activation of nitric oxide (NO) signalling pathway in the BMSCs. The data suggest that EPO-MNC-TGFβ1 could be one of the regulatory axes operative in the bone marrow microenvironment involved in maintaining the functionality of the resident BMSCs.
(© 2020 International Federation for Cell Biology.)
Databáze: MEDLINE
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