Comparison of DNA damage and proliferative capacities in smear samples of HPV positive and negative patients by micronucleus counting and AgNOR staining.

Autor: Sancer O; Department of Medical Biology, University of Süleyman Demirel, Isparta, Turkey., Şirin MC; Department of Medical Microbiology, University of Süleyman Demirel, Isparta, Turkey., Arıdoğan B; Department of Medical Microbiology, University of Süleyman Demirel, Isparta, Turkey., Sesli Çetin E; Department of Medical Microbiology, University of Süleyman Demirel, Isparta, Turkey., Yiğit A; Department of Medical Microbiology, University of Süleyman Demirel, Isparta, Turkey., Tepebaşı MY; Department of Medical Genetics, University of Süleyman Demirel, Isparta, Turkey., Aslan Koşar P; Department of Medical Biology, University of Süleyman Demirel, Isparta, Turkey.
Jazyk: angličtina
Zdroj: Biotechnic & histochemistry : official publication of the Biological Stain Commission [Biotech Histochem] 2021 Jul; Vol. 96 (5), pp. 354-358. Date of Electronic Publication: 2020 Aug 03.
DOI: 10.1080/10520295.2020.1800091
Abstrakt: Human papillomavirus (HPV) is believed to cause cervical cancer. Thousands of women develop cancer and other diseases caused by HPV each year. HPV 16 and 18 types are found in approximately 70% of cervical cancers. Micronuclei are small chromosomal fragments that are considered indicators of DNA damage. AgNOR positive dots are useful for assessing proliferation. We investigated the relation between HPV-DNA, micronuclei and AgNOR in smear samples. Three groups were defined: HPV negative, 16/18 positive and other high-risk groups (31, 33, 35, 39, 45, 51, 52, 56, 58, 66 and 68) (HR). After typing, micronuclei were identified by Papanicolaou staining and AgNOR regions were detected by silver staining. Serum reactive protein (CRP) also was measured. We found that the average age of HPV negative patients was significantly greater than for the HPV positive groups. We also found that CRP levels were significantly higher in the HPV 16/18 positive group than HPV negative and other HPV group. We found that the number of micronuclei in the HPV 16/18 group was significantly greater than for the HPV negative group. Also, we found that AgNOR staining for the HPV 16/18 group was significantly greater than for the HPV negative group. We found that CRP level, cell proliferation and genome instability were increased in HPV positive patients. The AgNOR and micronucleus tests were useful for evaluating cell proliferation and DNA damage.
Databáze: MEDLINE
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