| Autor: |
de Mendonça M; Departamento de Farmacologia, Universidade de Sao Paulo Instituto de Ciencias Biomedicas, São Paulo, Brasil., Rocha KC; Departamento de Farmacologia, Universidade de Sao Paulo Instituto de Ciencias Biomedicas, São Paulo, Brasil., de Sousa É; Departamento de Farmacologia, Universidade de Sao Paulo Instituto de Ciencias Biomedicas, São Paulo, Brasil., Pereira BMV; Departamento de Farmacologia, Universidade de Sao Paulo Instituto de Ciencias Biomedicas, São Paulo, Brasil., Oyama LM; Departamento de Fisiologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brasil., Rodrigues AC; Departamento de Farmacologia, Universidade de Sao Paulo Instituto de Ciencias Biomedicas, São Paulo, Brasil. |
| Jazyk: |
angličtina |
| Zdroj: |
American journal of physiology. Endocrinology and metabolism [Am J Physiol Endocrinol Metab] 2020 Sep 01; Vol. 319 (3), pp. E579-E591. Date of Electronic Publication: 2020 Aug 03. |
| DOI: |
10.1152/ajpendo.00172.2020 |
| Abstrakt: |
There is a growing body of evidence that extracellular vesicles (EVs) and their cargo of RNA, DNA, and protein are released in the circulation with exercise and might mediate interorgan communication. C57BL6/J male mice were subjected to diet-induced obesity and aerobic training on a treadmill for 8 wk. The effect of aerobic training was evaluated in the liver, muscle, kidney, and white/brown adipose tissue. To provide new mechanistic insight, we profiled miRNA from serum EVs of obese and obese trained mice. We demonstrate that aerobic training changes the circulating EV miRNA profile of obese mice, including decreases in miR-122, miR-192, and miR-22 levels. Circulating miRNA levels were associated with miRNA levels in mouse liver white adipose tissue (WAT). In WAT, aerobically trained obese mice showed reduced adipocyte hypertrophy and increased the number of smaller adipocytes and the expression of Cebpa , Pparg , Fabp4 (adipogenesis markers), and ATP-citrate lyase enzyme activity. Importantly, miR-22 levels negatively correlated with the expression of adipogenesis and insulin sensitivity markers. In the liver, aerobic training reverted obesity-induced steatohepatitis, and steatosis score and Pparg expression were negatively correlated with miR-122 levels. The prometabolic effects of aerobic exercise in obesity possibly involve EV miRNAs, which might be involved in communication between liver and WAT. Our data provide significant evidence demonstrating that aerobic training exercise-induced EVs mediate the effect of exercise on adipose tissue metabolism. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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