Meta-omics highlights the diversity, activity and adaptations of fungi in deep oceanic crust.

Autor: Quemener M; Université de Brest, EA 3882 Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, Technopôle Brest-Iroise, Plouzané, France., Mara P; Department of Geology and Geophysics, Woods Hole Oceanographic Institution, Woods Hole, MA, 02543, USA.; Department of Biology, Woods Hole Oceanographic Institution, Woods Hole, MA, 02543, USA., Schubotz F; MARUM-Center for Marine Environmental Sciences, University Bremen, Leobener Strasse 8, Bremen, 28359, Germany., Beaudoin D; Department of Geology and Geophysics, Woods Hole Oceanographic Institution, Woods Hole, MA, 02543, USA.; Department of Biology, Woods Hole Oceanographic Institution, Woods Hole, MA, 02543, USA., Li W; College of Marine Life Sciences, Ocean University of China, Qingdao, China., Pachiadaki M; Department of Geology and Geophysics, Woods Hole Oceanographic Institution, Woods Hole, MA, 02543, USA.; Department of Biology, Woods Hole Oceanographic Institution, Woods Hole, MA, 02543, USA., Sehein TR; Department of Geology and Geophysics, Woods Hole Oceanographic Institution, Woods Hole, MA, 02543, USA.; Department of Biology, Woods Hole Oceanographic Institution, Woods Hole, MA, 02543, USA., Sylvan JB; Department of Oceanography, Texas A&M University, College Station, TX, 77845, USA., Li J; State Key Laboratory of Marine Geology, Tongji University, Shanghai, 200092, China., Barbier G; Université de Brest, EA 3882 Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, Technopôle Brest-Iroise, Plouzané, France., Edgcomb V; Department of Geology and Geophysics, Woods Hole Oceanographic Institution, Woods Hole, MA, 02543, USA.; Department of Biology, Woods Hole Oceanographic Institution, Woods Hole, MA, 02543, USA., Burgaud G; Université de Brest, EA 3882 Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, Technopôle Brest-Iroise, Plouzané, France.
Jazyk: angličtina
Zdroj: Environmental microbiology [Environ Microbiol] 2020 Sep; Vol. 22 (9), pp. 3950-3967. Date of Electronic Publication: 2020 Aug 20.
DOI: 10.1111/1462-2920.15181
Abstrakt: The lithified oceanic crust, lower crust gabbros in particular, has remained largely unexplored by microbiologists. Recently, evidence for heterogeneously distributed viable and transcriptionally active autotrophic and heterotrophic microbial populations within low-biomass communities was found down to 750 m below the seafloor at the Atlantis Bank Gabbro Massif, Indian Ocean. Here, we report on the diversity, activity and adaptations of fungal communities in the deep oceanic crust from ~10 to 780 mbsf by combining metabarcoding analyses with mid/high-throughput culturing approaches. Metabarcoding along with culturing indicate a low diversity of viable fungi, mostly affiliated to ubiquitous (terrestrial and aquatic environments) taxa. Ecophysiological analyses coupled with metatranscriptomics point to viable and transcriptionally active fungal populations engaged in cell division, translation, protein modifications and other vital cellular processes. Transcript data suggest possible adaptations for surviving in the nutrient-poor, lithified deep biosphere that include the recycling of organic matter. These active communities appear strongly influenced by the presence of cracks and veins in the rocks where fluids and resulting rock alteration create micro-niches.
(© 2020 Society for Applied Microbiology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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