You get what you screen for: on the value of fermentation characterization in high-throughput strain improvements in industrial settings.

Autor: Wehrs M; Zymergen Inc., 5980 Horton Street, Suite #105, Emeryville, CA, 94608, USA., de Beaumont-Felt A; Zymergen Inc., 5980 Horton Street, Suite #105, Emeryville, CA, 94608, USA., Goranov A; Zymergen Inc., 5980 Horton Street, Suite #105, Emeryville, CA, 94608, USA., Harrigan P; Zymergen Inc., 5980 Horton Street, Suite #105, Emeryville, CA, 94608, USA., de Kok S; Zymergen Inc., 5980 Horton Street, Suite #105, Emeryville, CA, 94608, USA., Lieder S; Zymergen Inc., 5980 Horton Street, Suite #105, Emeryville, CA, 94608, USA., Vallandingham J; Zymergen Inc., 5980 Horton Street, Suite #105, Emeryville, CA, 94608, USA., Tyner K; Zymergen Inc., 5980 Horton Street, Suite #105, Emeryville, CA, 94608, USA. kris@zymergen.com.
Jazyk: angličtina
Zdroj: Journal of industrial microbiology & biotechnology [J Ind Microbiol Biotechnol] 2020 Nov; Vol. 47 (11), pp. 913-927. Date of Electronic Publication: 2020 Aug 02.
DOI: 10.1007/s10295-020-02295-3
Abstrakt: While design and high-throughput build approaches in biotechnology have increasingly gained attention over the past decade, approaches to test strain performance in high-throughput have received less discussion in the literature. Here, we describe how fermentation characterization can be used to improve the overall efficiency of high-throughput DBTAL (design-build-test-analyze-learn) cycles in an industrial context. Fermentation characterization comprises an in-depth study of strain performance in a bioreactor setting and involves semi-frequent sampling and analytical measurement of substrates, cell densities and viabilities, and (by)products. We describe how fermentation characterization can be used to (1) improve (high-throughput) strain design approaches; (2) enable the development of bench-scale fermentation processes compatible with a wide diversity of strains; and (3) inform the development of high-throughput plate-based strain testing procedures for improved performance at larger scales.
Databáze: MEDLINE
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