Dysregulated transcriptional responses to SARS-CoV-2 in the periphery support novel diagnostic approaches.

Autor:	McClain MT; Center for Applied Genomics and Precision Medicine, Duke University, Durham, NC.; Durham Veterans Affairs Medical Center, Durham, NC.; Division of Infectious Diseases, Duke University Medical Center, Durham, NC., Constantine FJ; Center for Applied Genomics and Precision Medicine, Duke University, Durham, NC., Henao R; Center for Applied Genomics and Precision Medicine, Duke University, Durham, NC., Liu Y; Center for Applied Genomics and Precision Medicine, Duke University, Durham, NC., Tsalik EL; Center for Applied Genomics and Precision Medicine, Duke University, Durham, NC.; Durham Veterans Affairs Medical Center, Durham, NC.; Division of Infectious Diseases, Duke University Medical Center, Durham, NC., Burke TW; Center for Applied Genomics and Precision Medicine, Duke University, Durham, NC., Steinbrink JM; Center for Applied Genomics and Precision Medicine, Duke University, Durham, NC., Petzold E; Center for Applied Genomics and Precision Medicine, Duke University, Durham, NC., Nicholson BP; institute for Medical Research, Durham, NC., Rolfe R; Division of Infectious Diseases, Duke University Medical Center, Durham, NC., Kraft BD; Division of Pulmonary, Allergy, and Critical Care Medicine, Duke University Medical Center, Durham, NC., Kelly MS; Division of Pediatric Infectious Diseases, Duke University Medical Center., Sempowski GD; Duke Human Vaccine Institute, Durham, NC., Denny TN; Duke Human Vaccine Institute, Durham, NC., Ginsburg GS; Center for Applied Genomics and Precision Medicine, Duke University, Durham, NC., Woods CW; Center for Applied Genomics and Precision Medicine, Duke University, Durham, NC.; Durham Veterans Affairs Medical Center, Durham, NC.; Division of Infectious Diseases, Duke University Medical Center, Durham, NC.
Jazyk:	angličtina
Zdroj:	MedRxiv : the preprint server for health sciences [medRxiv] 2020 Jul 26. Date of Electronic Publication: 2020 Jul 26.
DOI:	10.1101/2020.07.20.20155507
Abstrakt:	In order to elucidate novel aspects of the host response to SARS-CoV-2 we performed RNA sequencing on peripheral blood samples across 77 timepoints from 46 subjects with COVID-19 and compared them to subjects with seasonal coronavirus, influenza, bacterial pneumonia, and healthy controls. Early SARS-CoV-2 infection triggers a conserved transcriptomic response in peripheral blood that is heavily interferon-driven but also marked by indicators of early B-cell activation and antibody production. Interferon responses during SARS-CoV-2 infection demonstrate unique patterns of dysregulated expression compared to other infectious and healthy states. Heterogeneous activation of coagulation and fibrinolytic pathways are present in early COVID-19, as are IL1 and JAK/STAT signaling pathways, that persist into late disease. Classifiers based on differentially expressed genes accurately distinguished SARS-CoV-2 infection from other acute illnesses (auROC 0.95). The transcriptome in peripheral blood reveals unique aspects of the immune response in COVID-19 and provides for novel biomarker-based approaches to diagnosis.
Databáze:	MEDLINE
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