Unraveling the molecular pathobiology of vocal fold systemic dehydration using an in vivo rabbit model.

Autor: Cannes do Nascimento N; Department of Speech, Language, and Hearing Sciences, Purdue University, West Lafayette, Indiana, United States of America., Dos Santos AP; Department of Comparative Pathobiology, Purdue University, West Lafayette, Indiana, United States of America., Sivasankar MP; Department of Speech, Language, and Hearing Sciences, Purdue University, West Lafayette, Indiana, United States of America., Cox A; Department of Comparative Pathobiology, Purdue University, West Lafayette, Indiana, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2020 Jul 31; Vol. 15 (7), pp. e0236348. Date of Electronic Publication: 2020 Jul 31 (Print Publication: 2020).
DOI: 10.1371/journal.pone.0236348
Abstrakt: Vocal folds are a viscoelastic multilayered structure responsible for voice production. Vocal fold epithelial damage may weaken the protection of deeper layers of lamina propria and thyroarytenoid muscle and impair voice production. Systemic dehydration can adversely affect vocal function by creating suboptimal biomechanical conditions for vocal fold vibration. However, the molecular pathobiology of systemically dehydrated vocal folds is poorly understood. We used an in vivo rabbit model to investigate the complete gene expression profile of systemically dehydrated vocal folds. The RNA-Seq based transcriptome revealed 203 differentially expressed (DE) vocal fold genes due to systemic dehydration. Interestingly, function enrichment analysis showed downregulation of genes involved in cell adhesion, cell junction, inflammation, and upregulation of genes involved in cell proliferation. RT-qPCR validation was performed for a subset of DE genes and confirmed the downregulation of DSG1, CDH3, NECTIN1, SDC1, S100A9, SPINK5, ECM1, IL1A, and IL36A genes. In addition, the upregulation of the transcription factor NR4A3 gene involved in epithelial cell proliferation was validated. Taken together, these results suggest an alteration of the vocal fold epithelial barrier independent of inflammation, which could indicate a disruption and remodeling of the epithelial barrier integrity. This transcriptome provides a first global picture of the molecular changes in vocal fold tissue in response to systemic dehydration. The alterations observed at the transcriptional level help to understand the pathobiology of dehydration in voice function and highlight the benefits of hydration in voice therapy.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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