Inhibitory Effects of Euphorbia tirucalli Lineu (Euphorbiaceae) Diluted Latex on Human and Canine Melanoma Cells.

Autor: Alves da Paz DP; School of Veterinary Medicine and Animal Science of the University of São Paulo, Laboratory of Experimental and Comparative Oncology, Department of Pathology, Av Prof. Dr. Orlando Marques de Paiva, 87 CEP 05508-900, São Paulo, SP, Brazil., Nagamine MK; School of Veterinary Medicine and Animal Science of the University of São Paulo, Laboratory of Experimental and Comparative Oncology, Department of Pathology, Av Prof. Dr. Orlando Marques de Paiva, 87 CEP 05508-900, São Paulo, SP, Brazil., Grande MPD; School of Veterinary Medicine and Animal Science of the University of São Paulo, Laboratory of Experimental and Comparative Oncology, Department of Pathology, Av Prof. Dr. Orlando Marques de Paiva, 87 CEP 05508-900, São Paulo, SP, Brazil., Leite JVP; School of Veterinary Medicine and Animal Science of the University of São Paulo, Laboratory of Experimental and Comparative Oncology, Department of Pathology, Av Prof. Dr. Orlando Marques de Paiva, 87 CEP 05508-900, São Paulo, SP, Brazil., Sobreira FMG; School of Pharmaceutical Sciences of the University of São Paulo, Department of Pharmacy, Av. Professor Lineu Prestes, 580 CEP 05508-000, São Paulo, SP, Brazil., Bacchi EM; School of Pharmaceutical Sciences of the University of São Paulo, Department of Pharmacy, Av. Professor Lineu Prestes, 580 CEP 05508-000, São Paulo, SP, Brazil., Zaidan Dagli ML; School of Veterinary Medicine and Animal Science of the University of São Paulo, Laboratory of Experimental and Comparative Oncology, Department of Pathology, Av Prof. Dr. Orlando Marques de Paiva, 87 CEP 05508-900, São Paulo, SP, Brazil.
Jazyk: angličtina
Zdroj: Evidence-based complementary and alternative medicine : eCAM [Evid Based Complement Alternat Med] 2020 Jul 14; Vol. 2020, pp. 4093206. Date of Electronic Publication: 2020 Jul 14 (Print Publication: 2020).
DOI: 10.1155/2020/4093206
Abstrakt: Euphorbia tirucalli Lineu (Euphorbiaceae) is a tropical and subtropical ornamental and toxic plant. E. tirucalli produces a latex that is commonly used to treat neoplasms. This study aimed to evaluate the effects of diluted E. tirucalli latex (DETL) on human (SK-MEL-28) and canine (CBMY) melanoma cells. SK-MEL-28 (3 × 10 3  cells/well) and CBMY (6 × 10 3  cells/well) were cultivated in 96-well plates. The cells were treated with 50  μ l/well of dilutions (1/2, 1/4, 1/8, 1/16, 1/32, 1/64, 1/128, 1/256, and 1/512) of a standard solution containing 1 mg/mL of the E. tirucalli latex (ETL) in DMEM. Control group cells received 50  μ l/well of DMEM. After 24, 48, and 72 h of treatment, cell viability was assessed by the MTT assay. There was a significant decrease in viability at 48 and 72 hours after treatment for human melanoma cells and at 24, 48, and 72 hours for canine cells, mainly in higher dilutions of ETL. Human melanoma cells presented a typical U shape curve, characteristic of hormesis. To our knowledge, this is the first study showing inhibitory effects of DETL on canine melanoma cells. Therefore, DETL is a potentially new antineoplastic drug.
Competing Interests: The authors declare no conflicts of interest.
(Copyright © 2020 Diego Pinha Alves da Paz et al.)
Databáze: MEDLINE
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