Dual expression of transgenic delta-5 and delta-6 desaturase in tilapia alters gut microbiota and enhances resistance to Vibrio vulnificus infection.

Autor: Chiang KY; Department of Life Science, National Taiwan University, Taipei, Taiwan.; Institute of Cellular and Organismic Biology, Academia Sinica, Nankang, Taipei, Taiwan., Lin WC; Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, Ilan, Taiwan., Tsai TY; Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, Ilan, Taiwan., Lin CW; Institute of Cellular and Organismic Biology, Academia Sinica, Nankang, Taipei, Taiwan., Huang SJ; Institute of Cellular and Organismic Biology, Academia Sinica, Nankang, Taipei, Taiwan., Huang CY; Institute of Cellular and Organismic Biology, Academia Sinica, Nankang, Taipei, Taiwan., Wu SH; Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan., Ken CF; Institute of Biotechnology, National Changhua University of Education, Changhua, Taiwan., Gong HY; Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan.; Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung, Taiwan., Chen JY; Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, Ilan, Taiwan., Wu JL; Institute of Cellular and Organismic Biology, Academia Sinica, Nankang, Taipei, Taiwan.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2020 Jul 30; Vol. 15 (7), pp. e0236601. Date of Electronic Publication: 2020 Jul 30 (Print Publication: 2020).
DOI: 10.1371/journal.pone.0236601
Abstrakt: Omega-3 polyunsaturated fatty acids (n-3 PUFAs), such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), exhibit antibacterial and anti-inflammatory activities. Furthermore, diets rich in n-3 PUFAs are known to improve disease resistance and limit pathogen infection in commercial aquaculture fishes. In this study, we examined the effects of transgenic overexpression of n-3 PUFA biosynthesis genes on the physiological response to bacterial infection in tilapia. We first established tilapia strains with single or dual expression of salmon delta-5 desaturase and/or delta-6 desaturase and then challenged the fish with Vibrio vulnificus infection. Interestingly, our data suggest that n-3 PUFA-mediated alterations in gut microbiota may be important in determining disease outcome via effects on immune response of the host. Both liver- and muscle-specific single and dual expression of delta-5 desaturase and delta-6 desaturase resulted in higher n-3 PUFA content in transgenic fish fed with a LO basal diet. The enrichment of n-3 PUFAs in dual-transgenic fish is likely responsible for their improved survival rate and comparatively reduced expression of inflammation- and immune-associated genes after V. vulnificus infection. Gut microbiome analysis further revealed that dual-transgenic tilapia had high gut microbiota diversity, with low levels of inflammation-associated microbiota (i.e., Prevotellaceae). Thus, our findings indicate that dual expression of transgenic delta-5 and delta-6 desaturase in tilapia enhances disease resistance, an effect that is associated with increased levels of n-3 PUFAs and altered gut microbiota composition.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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