| Autor: |
Roque-Jiménez JA; Facultad de Agronomía y Veterinaria, Universidad Autonoma de San Luis Potosí, Carretera Federal 57 Km 14.5, Ejido Palma de la Cruz, Soledad de Graciano Sánchez, San Luis Potosí 78321, Mexico., Mendoza-Martínez GD; Departamento de Producción Animal, Universidad Autonoma Metropolitana-Xochimilco, CDMX, Mexico City 04960, Mexico., Vázquez-Valladolid A; Facultad de Agronomía y Veterinaria, Universidad Autonoma de San Luis Potosí, Carretera Federal 57 Km 14.5, Ejido Palma de la Cruz, Soledad de Graciano Sánchez, San Luis Potosí 78321, Mexico., Guerrero-González ML; Facultad de Agronomía y Veterinaria, Universidad Autonoma de San Luis Potosí, Carretera Federal 57 Km 14.5, Ejido Palma de la Cruz, Soledad de Graciano Sánchez, San Luis Potosí 78321, Mexico., Flores-Ramírez R; Centro de Investigación Aplicada en Ambiente y Salud, CIACYT-Medicina, Universidad Autonoma de San Luis Potosí, Lomas de San Luis 78210, Mexico., Pinos-Rodriguez JM; Facultad de Medicina Veterinaria y Zootecnia, Universidad Veracruzana, Veracruz 91710, Mexico., Loor JJ; Department of Animal Sciences, Division of Nutritional Sciences, University of Illinois, 262 Animal Sciences Laboratory, Urbana, IL 61801, USA., Relling AE; Department of Animal Science, The Ohio State University, Ohio Agricultural Research and Development Center (OARDC), Wooster, OH 44691, USA., Lee-Rangel HA; Facultad de Agronomía y Veterinaria, Universidad Autonoma de San Luis Potosí, Carretera Federal 57 Km 14.5, Ejido Palma de la Cruz, Soledad de Graciano Sánchez, San Luis Potosí 78321, Mexico. |
| Abstrakt: |
Herbal formulas during pregnancy have been used in developing countries. Despite that, the potential effects on the mother and offspring and whether those supplements elicit epigenetic modifications is still unknown. Therefore, our objectives were to determine the effects of supplemental herbal choline source (BCho) on the percentage of 5-hmC in whole blood from gestating ewes and their offspring, as well as determining the milk quality and growth of the offspring. Thirty-five gestating Rambouillet ewes were randomly assigned to five treatments: T1, supplementation of 4 g per day (gd -1 ) of BCho during the first third of gestation; T2, supplementation of 4 gd -1 of BCho during the second third of gestation; T3, supplementation of 4 gd -1 of BCho during the last third of gestation; T4, supplementation of 4 gd -1 of BCho throughout gestation; and T5, no BCho supplementation (control). For the 5-hmC DNA analysis, whole blood from ewes was sampled before pregnancy and at each third of gestation (50 days). Whole blood from lambs was sampled five weeks after birth. The evaluation of the nutritional programming effects was conducted through the percentages of 5-hmC in the lambs. Compared with other treatments, the whole blood from ewes supplemented during T1 and T4 had the greatest 5-hmC percentages ( p < 0.05). However, only ewes fed BCho throughout gestation (T4) maintained the greatest percentages of 5-hmC ( p < 0.05). The lamb growth performance indicated that the BCho maternal supplementation did not affect the nutritional programming. However, the lambs born from ewes supplemented during T2 had the greatest 5-hmC percentages ( p < 0.05). Our data suggest that ewes supplemented during T4 with BCho increase and maintain the percentages of 5-hmC in whole blood, and the offspring born from ewes supplemented with BCho during T2 maintained the greatest percentages of 5-hmC 35 d after they were born. |
