Macroscopic, biochemical and hystological evaluation of topical anti-inflammatory activity of Casearia sylvestris (Flacourtiaceae) in mice.

Autor: Heymanns AC; Post-graduate Programm in Health Science, University of Southern Catarina (UNISUL), Tubarão, SC, Brazil; Laboratory of Experimental Neuroscience (LANEX)-UNISUL, Palhoça, SC, Brazil. Electronic address: nutriheymanns@gmail.com., Albano MN; Pharmacy Degree Course UNISUL, Tubarão, SC, Brazil. Electronic address: micheline.albano@gmail.com., da Silveira MR; Pharmacy Degree Course UNISUL, Tubarão, SC, Brazil. Electronic address: marcia15_rs@hotmail.com., Muller SD; Pharmacy Degree Course UNISUL, Tubarão, SC, Brazil. Electronic address: simony.davet@unisul.br., Petronilho FC; Post-graduate Programm in Health Science, University of Southern Catarina (UNISUL), Tubarão, SC, Brazil. Electronic address: fabricia.petronilho@unisul.br., Gainski LD; Post-graduate Programm in Health Science, University of Southern Catarina (UNISUL), Tubarão, SC, Brazil. Electronic address: lucineia.gainski@unisul.br., Cargnin-Ferreira E; Laboratory of Histological Markers, Federal Institute of Santa Catarina (IFSC), Garopaba, SC, Brazil. Electronic address: eduardo.cargnin.ferreira@gmail.com., Piovezan AP; Post-graduate Programm in Health Science, University of Southern Catarina (UNISUL), Tubarão, SC, Brazil; Laboratory of Experimental Neuroscience (LANEX)-UNISUL, Palhoça, SC, Brazil. Electronic address: appiovezan@yahoo.com.br.
Jazyk: angličtina
Zdroj: Journal of ethnopharmacology [J Ethnopharmacol] 2021 Jan 10; Vol. 264, pp. 113139. Date of Electronic Publication: 2020 Jul 26.
DOI: 10.1016/j.jep.2020.113139
Abstrakt: Ethnopharmacological Relevance: Inflammatory skin diseases presents high prevalence and lack of alternatives that can be used for self-care by the population. Casearia sylvestris is a plant used topically in different communities in Brazil, to treat wounds or promote cutaneous healing. To evaluate the topical anti-inflammatory activity for the crude hydroalcoholic extract of Casearia sylvestris (HCE-CS) in the models of single or multiple administration of chroton oil to induce ear edema in mice.
Materials and Methods: Experimental study using male Swiss mice (25-35g) kept under constant conditions in the Laboratory of Experimental Neuroscience (LaNEx)-UNISUL. Edema was induced in both models, respectively, by the single or multiple application of croton oil (CO, 2.5%, in 20 μl) on the external surface of the ear. The different groups of animals (n = 8) received different treatments: vehicle, dexamethasone (DEXA) or different doses of HCE-CS. Edema was evaluated macroscopically for 6 h (early edema) or 8 days (late edema) after the first application of the CO and immediately after the animals were submitted to euthanasia for the collection of the samples (treated ears). For early edema, the tissue was biochemically evaluated for myeloperoxidase activity (MPO) and levels of nitrite/nitrate. In the late edema model, the ears were histologically evaluated for general morphometry, degranulated and non-degranulated mast cells, as well as acanthosis.
Results: Topic treatment with HCE-CS significantly reduced the early and late edema, as well as MPO activity and tissue levels of nitrite/nitrate. Finally, in the late edema model there was a lower density of degranulated mast cells in relation to the vehicle treated group and decreased thickness of the epidermis (acanthosis).
Conclusion: These results suggest a possible benefit of topical treatment with HCE-CS in inflammatory conditions of the skin.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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