Modulation of lipase B from Candida antarctica properties via covalent immobilization on eco-friendly support for enzymatic kinetic resolution of rac-indanyl acetate.

Autor: de Souza TC; Departamento de Engenharia Química, Universidade Federal do Ceará, Campus do Pici, Fortaleza, CE, CEP 60455760, Brazil., de Sousa Fonseca T; Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, Campus do Pici, Fortaleza, CE, CEP 60455970, Brazil., de Sousa Silva J; Departamento de Engenharia Química, Universidade Federal do Ceará, Campus do Pici, Fortaleza, CE, CEP 60455760, Brazil., Lima PJM; Departamento de Engenharia Química, Universidade Federal do Ceará, Campus do Pici, Fortaleza, CE, CEP 60455760, Brazil., Neto CACG; Departamento de Engenharia Química, Universidade Federal do Ceará, Campus do Pici, Fortaleza, CE, CEP 60455760, Brazil., Monteiro RRC; Departamento de Engenharia Química, Universidade Federal do Ceará, Campus do Pici, Fortaleza, CE, CEP 60455760, Brazil., Rocha MVP; Departamento de Engenharia Química, Universidade Federal do Ceará, Campus do Pici, Fortaleza, CE, CEP 60455760, Brazil., de Mattos MC; Departamento de Engenharia Química, Universidade Federal do Ceará, Campus do Pici, Fortaleza, CE, CEP 60455760, Brazil., Dos Santos JCS; Departamento de Engenharia Química, Universidade Federal do Ceará, Campus do Pici, Fortaleza, CE, CEP 60455760, Brazil. jcs@unilab.edu.br.; Instituto de Engenharias e Desenvolvimento Sustentável, Universidade da Integração Internacional da Lusofonia Afro-Brasileira, Redenção, CE, CEP 62785000, Brazil. jcs@unilab.edu.br., Gonçalves LRB; Departamento de Engenharia Química, Universidade Federal do Ceará, Campus do Pici, Fortaleza, CE, CEP 60455760, Brazil. lrg@ufc.br.
Jazyk: angličtina
Zdroj: Bioprocess and biosystems engineering [Bioprocess Biosyst Eng] 2020 Dec; Vol. 43 (12), pp. 2253-2268. Date of Electronic Publication: 2020 Jul 28.
DOI: 10.1007/s00449-020-02411-8
Abstrakt: In this study, the modulation of enzymatic biocatalysts were developed by the use of lipase B from Candida antarctica covalently immobilized on an eco-friendly support, cashew apple bagasse, activated with 10% glycidol-ethylenediamine-glutaraldehyde (GEG) under different immobilization strategies (5 mM or 100 mM ionic strength and in absence or presence of 0.5% (v/v) Triton X-100). The biocatalysts were characterized for thermal and organic solvents stabilities and compared with the soluble enzyme. The biocatalysts were then applied to the hydrolysis of the rac-indanyl acetate (2:1 ratio enzyme/substrate) at pH 7.0 and 30 °C for 24 h. For all the strategies evaluated, GEG promoted kinetic resolution of rac-indanyl acetate with maximum conversion (50%) and led to (R)-indanol with excellent enantiomeric excess (97%), maintaining the maximum conversion for five consecutive cycles of hydrolysis. Therefore, the use of cashew apple bagasse has proved to be a promising eco-friendly support for enzyme immobilization, since it resulted in stable biocatalysts for enzymatic kinetic resolution.
Databáze: MEDLINE
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