Cervical Cytology of Samples with Ureaplasma urealyticum , Ureaplasma parvum , Chlamydia trachomatis , Trichomonas vaginalis , Mycoplasma hominis , and Neisseria gonorrhoeae Detected by Multiplex PCR.

Autor: Carneiro FP; Pathology Department of Brasília University, Brasília, Federal District, Brazil.; Catholic University of Brasilia, Brasília, Federal District, Brazil., Darós AC; Pathology Department of Brasília University, Brasília, Federal District, Brazil., Darós ACM; Pathology Department of Brasília University, Brasília, Federal District, Brazil., de Castro TMML; Pathology Department of Brasília University, Brasília, Federal District, Brazil., de Vasconcelos Carneiro M; Catholic University of Brasilia, Brasília, Federal District, Brazil., Fidelis CR; Gynecological Unit, University Hospital of Brasilia, Brasília, Federal District, Brazil., Vilioni MV; Pathology Department of Brasília University, Brasília, Federal District, Brazil., da Costa Matsunaga ME; Gynecological Unit, University Hospital of Brasilia, Brasília, Federal District, Brazil., Sidou JMO; Gynecological Unit, University Hospital of Brasilia, Brasília, Federal District, Brazil., Chaves MALD; Gynecological Unit, University Hospital of Brasilia, Brasília, Federal District, Brazil., Pereira LC; Gynecological Unit, University Hospital of Brasilia, Brasília, Federal District, Brazil., de Resende CN; Gynecological Unit, University Hospital of Brasilia, Brasília, Federal District, Brazil., de Castro Moreira Dos Santos A; Molecular Biology Laboratory-LACEN DF, Brazil., Ferreira VM; Pathology Department of Brasília University, Brasília, Federal District, Brazil., Motoyama AB; Pathology Department of Brasília University, Brasília, Federal District, Brazil.
Jazyk: angličtina
Zdroj: BioMed research international [Biomed Res Int] 2020 Jul 07; Vol. 2020, pp. 7045217. Date of Electronic Publication: 2020 Jul 07 (Print Publication: 2020).
DOI: 10.1155/2020/7045217
Abstrakt: Introduction: Despite increasing application of molecular diagnostic methods for the detection of sexually transmitted infections, the cytological findings in pap smears of patients with pathogens that can be identified only by PCR are not yet well described. The aim of this study was to describe the most common cytological features in cervical pap smears of patients with Chlamydia trachomatis , Neisseria gonorrhoeae , Mycoplasma genitalium , Trichomonas vaginalis , Mycoplasma hominis , Ureaplasma urealyticum , and Ureaplasma parvum detected by multiplex PCR.
Methods: Cervical samples for conventional and liquid-based cytology and for multiplex PCR were collected from women ranging from 23 to 54 years old, who underwent routine screening at a gynecological Unit.
Results: Multiplex PCR was positive in 36.2% of the samples: Ureaplasma parvum 14.9%, Chlamydia trachomatis 10.6%, Trichomonas vaginalis 10.6%, Mycoplasma hominis 8.5%, Ureaplasma urealyticum 4.2%, Neisseria gonorrhoeae 2.1%, and Mycoplasma genitalium (0). Multiple pathogens were observed in 12.8% of samples. Microscopic cervicitis (≥10 polymorphonuclear leukocytes/epithelial cell) and normal (predominantly lactobacillary) microbiota were the most frequent findings in the samples in which the pathogens were detected alone or in multiple infections, except for samples with Trichomonas vaginalis in which the coccobacillary microbiota was the most common. In samples with microscopic cervicitis and normal microbiota, those with at least one pathogen identified by multiplex PCR were significantly more frequent than those with no pathogen, 66.6% versus 33.3%.
Conclusion: Failure to identify an inflammatory agent in pap smear with intense neutrophil exudate may suggest the presence of Ureaplasma parvum , Ureaplasma urealyticum , Chlamydia trachomatis , or Trichomonas vaginalis . A remark on the intensity of inflammation should be made in the reports of cervical pap smears so that this cytological finding can be correlated with clinical and PCR results.
Competing Interests: All authors declare no conflicts of interest.
(Copyright © 2020 Fabiana Pirani Carneiro et al.)
Databáze: MEDLINE
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