| Autor: |
N Nuñez LF; Department of Pathology, School of Veterinary Medicine, University of São Paulo (USP), Av. Prof. Dr. Orlando M. Paiva, 87, São Paulo CEP 05508-270, SP, Brazil.; Facultad de Ciencias de la Salud, Carrera de Medicina Veterinaria, Universidad de Las Américas (UDLA), Av. Jose Queri, Quito 170513, Ecuador., Santander-Parra SH; Department of Pathology, School of Veterinary Medicine, University of São Paulo (USP), Av. Prof. Dr. Orlando M. Paiva, 87, São Paulo CEP 05508-270, SP, Brazil., De la Torre DI; Department of Pathology, School of Veterinary Medicine, University of São Paulo (USP), Av. Prof. Dr. Orlando M. Paiva, 87, São Paulo CEP 05508-270, SP, Brazil.; Institute for Research in Biomedicine, Central University of Ecuador, Quito CP E170201, Ecuador., Sá LRM; Department of Pathology, School of Veterinary Medicine, University of São Paulo (USP), Av. Prof. Dr. Orlando M. Paiva, 87, São Paulo CEP 05508-270, SP, Brazil., Buim MR; Laboratory of Avian Diseases, Instituto Biológico, Av. Gaspar Ricardo, 1700, Bastos CEP 17690-000, SP, Brazil., Astolfi-Ferreira CS; Department of Pathology, School of Veterinary Medicine, University of São Paulo (USP), Av. Prof. Dr. Orlando M. Paiva, 87, São Paulo CEP 05508-270, SP, Brazil., Piantino Ferreira AJ; Department of Pathology, School of Veterinary Medicine, University of São Paulo (USP), Av. Prof. Dr. Orlando M. Paiva, 87, São Paulo CEP 05508-270, SP, Brazil. |
| Abstrakt: |
Chicken parvovirus (ChPV) is an agent frequently associated with runting stunting syndrome (RSS). This syndrome has been reported in association with ChPV in many countries, including Brazil; however, studies characterizing the virus on a molecular level are scarce, and ChPV pathogenicity in day-old chicks remains unclear. The aim of the present work was to establish the molecular characteristics of ChPV, determine the pathogenicity of ChPV in SPF chicks and detect and quantify ChPV by qPCR in several tissues and chicks of different ages. The experimental challenge was performed at one day of age, and daily and weekly observations were performed and five birds from each experimental group (mock and infected birds) were euthanized to perform the different analysis. ChPV genome copies were detected and quantified by qPCR in gut, spleen, thymus, kidney, pancreas, proventriculus and bursa. Clinically, the infected group presented with diarrhea 24 h post-infection, which persisted until 42 days of age. The small intestine was distended, and its contents were aqueous and foamy. Enteritis and dilated crypts with cyst shapes were observed in intestinal segments. Acute pancreatitis associated with lymphocytic nodules, infiltrating lymphocytes and plasma cells between the pancreatic acinus was observed. Koch's postulate was demonstrated and the genetic characterization of the VP1 gene showed that the Brazilian ChPV isolate belongs to the ChPV II group. |
