Isolation and phylogenetic analysis of SARS-CoV-2 variants collected in Russia during the COVID-19 outbreak.

Autor:	Kozlovskaya L; FSBSI 'Chumakov Federal Scientific Center for Research and Development of Immune and Biological Products of the Russian Academy of Sciences' (FSBSI 'Chumakov FSC R&D IBP RAS'), Moscow 108819, Russia; Sechenov Moscow State Medical University, Moscow 119991, Russia. Electronic address: lubov_i_k@mail.ru., Piniaeva A; FSBSI 'Chumakov Federal Scientific Center for Research and Development of Immune and Biological Products of the Russian Academy of Sciences' (FSBSI 'Chumakov FSC R&D IBP RAS'), Moscow 108819, Russia., Ignatyev G; FSBSI 'Chumakov Federal Scientific Center for Research and Development of Immune and Biological Products of the Russian Academy of Sciences' (FSBSI 'Chumakov FSC R&D IBP RAS'), Moscow 108819, Russia., Selivanov A; FSBSI 'Chumakov Federal Scientific Center for Research and Development of Immune and Biological Products of the Russian Academy of Sciences' (FSBSI 'Chumakov FSC R&D IBP RAS'), Moscow 108819, Russia., Shishova A; FSBSI 'Chumakov Federal Scientific Center for Research and Development of Immune and Biological Products of the Russian Academy of Sciences' (FSBSI 'Chumakov FSC R&D IBP RAS'), Moscow 108819, Russia; Sechenov Moscow State Medical University, Moscow 119991, Russia., Kovpak A; FSBSI 'Chumakov Federal Scientific Center for Research and Development of Immune and Biological Products of the Russian Academy of Sciences' (FSBSI 'Chumakov FSC R&D IBP RAS'), Moscow 108819, Russia., Gordeychuk I; FSBSI 'Chumakov Federal Scientific Center for Research and Development of Immune and Biological Products of the Russian Academy of Sciences' (FSBSI 'Chumakov FSC R&D IBP RAS'), Moscow 108819, Russia; Sechenov Moscow State Medical University, Moscow 119991, Russia., Ivin Y; FSBSI 'Chumakov Federal Scientific Center for Research and Development of Immune and Biological Products of the Russian Academy of Sciences' (FSBSI 'Chumakov FSC R&D IBP RAS'), Moscow 108819, Russia., Berestovskaya A; GKB No. 40 DZM, Moscow 129301, Russia., Prokhortchouk E; NRC 'Kurchatov Institute', Moscow 123182, Russia., Protsenko D; GKB No. 40 DZM, Moscow 129301, Russia., Rychev M; NRC 'Kurchatov Institute', Moscow 123182, Russia., Ishmukhametov A; FSBSI 'Chumakov Federal Scientific Center for Research and Development of Immune and Biological Products of the Russian Academy of Sciences' (FSBSI 'Chumakov FSC R&D IBP RAS'), Moscow 108819, Russia; Sechenov Moscow State Medical University, Moscow 119991, Russia.
Jazyk:	angličtina
Zdroj:	International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases [Int J Infect Dis] 2020 Oct; Vol. 99, pp. 40-46. Date of Electronic Publication: 2020 Jul 25.
DOI:	10.1016/j.ijid.2020.07.024
Abstrakt:	Objectives: The outbreak of coronavirus disease 2019 (COVID-19) started in December 2019 in China and then spread worldwide over the following months, involving 188 countries. The objective of this study was to determine the molecular epidemiology of the COVID-19 outbreak in Russia. Methods: In this study, two severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) strains were isolated and genetically characterized. A phylogenetic analysis of all available Russian sequences was then performed and these were compared to the epidemiological data on COVID-19 incidence to evaluate the molecular epidemiology and pattern of virus spread in the territory of Russia. Results and Conclusions: Whole genome analysis of the isolates obtained in this study and 216 others isolated in Russia revealed a set of seven common mutations when compared to the original Wuhan virus, including amino acid substitutions in spike protein S and nucleoprotein N, possibly affecting their properties. Phylogenetic analysis of all Russian sequences and 8717 sequences from other countries showed multiple importations of the virus into Russia, local circulation, and several patterns of virus spread. (Copyright © 2020 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
Databáze:	MEDLINE
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