Isolating live cell clones from barcoded populations using CRISPRa-inducible reporters.

Autor: Umkehrer C; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria., Holstein F; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria., Formenti L; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria., Jude J; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria., Froussios K; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria., Neumann T; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria., Cronin SM; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria., Haas L; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria., Lipp JJ; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria.; Boehringer Ingelheim RCV GmbH & Co. KG, Vienna, Austria., Burkard TR; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria., Fellner M; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria., Wiesner T; Department of Dermatology, Medical University of Vienna, Vienna, Austria., Zuber J; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria.; Medical University of Vienna, Vienna BioCenter (VBC), Vienna, Austria., Obenauf AC; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria. anna.obenauf@imp.ac.at.
Jazyk: angličtina
Zdroj: Nature biotechnology [Nat Biotechnol] 2021 Feb; Vol. 39 (2), pp. 174-178. Date of Electronic Publication: 2020 Jul 27.
DOI: 10.1038/s41587-020-0614-0
Abstrakt: We developed a functional lineage tracing tool termed CaTCH (CRISPRa tracing of clones in heterogeneous cell populations). CaTCH combines precise clonal tracing of millions of cells with the ability to retrospectively isolate founding clones alive before and during selection, allowing functional experiments. Using CaTCH, we captured rare clones representing as little as 0.001% of a population and investigated the emergence of resistance to targeted melanoma therapy in vivo.
Databáze: MEDLINE
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