A Meta-Analysis of Wolbachia Transcriptomics Reveals a Stage-Specific Wolbachia Transcriptional Response Shared Across Different Hosts.

Autor: Chung M; Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD 21201.; Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD 21201., Basting PJ; Institute of Bioinformatics, University of Georgia, Athens, GA 30602., Patkus RS; Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD 21201., Grote A; Department of Biology, Center for Genomics and Systems Biology, New York University, NY 10003., Luck AN; New England Biolabs, Ipswich, MA 01938., Ghedin E; Department of Biology, Center for Genomics and Systems Biology, New York University, NY 10003., Slatko BE; New England Biolabs, Ipswich, MA 01938., Michalski M; Department of Biology, University of Wisconsin Oshkosh, WI 54901., Foster JM; New England Biolabs, Ipswich, MA 01938., Bergman CM; Institute of Bioinformatics, University of Georgia, Athens, GA 30602.; Department of Genetics, University of Georgia, Athens, GA 30602., Hotopp JCD; Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD 21201 jdhotopp@som.umaryland.edu.; Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD 21201.; Greenebaum Cancer Center, University of Maryland, Baltimore, MD 21201.
Jazyk: angličtina
Zdroj: G3 (Bethesda, Md.) [G3 (Bethesda)] 2020 Sep 02; Vol. 10 (9), pp. 3243-3260. Date of Electronic Publication: 2020 Sep 02.
DOI: 10.1534/g3.120.401534
Abstrakt: Wolbachia is a genus containing obligate, intracellular endosymbionts with arthropod and nematode hosts. Numerous studies have identified differentially expressed transcripts in Wolbachia endosymbionts that potentially inform the biological interplay between these endosymbionts and their hosts, albeit with discordant results. Here, we re-analyze previously published Wolbachia RNA-Seq transcriptomics data sets using a single workflow consisting of the most up-to-date algorithms and techniques, with the aim of identifying trends or patterns in the pan- Wolbachia transcriptional response. We find that data from one of the early studies in filarial nematodes did not allow for robust conclusions about Wolbachia differential expression with these methods, suggesting the original interpretations should be reconsidered. Across datasets analyzed with this unified workflow, there is a general lack of global gene regulation with the exception of a weak transcriptional response resulting in the upregulation of ribosomal proteins in early larval stages. This weak response is observed across diverse Wolbachia strains from both nematode and insect hosts suggesting a potential pan- Wolbachia transcriptional response during host development that diverged more than 700 million years ago.
(Copyright © 2020 Chung et al.)
Databáze: MEDLINE
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