Enhancement of photodynamic inactivation of planktonic cultures of Staphylococcus aureus by DMMB-AuNPs.

Autor: Monteiro JSC; Department of Biology, Feira de Santana State University, Feira de Santa, BA, CEP 44036-900, Brazil; Center of Biophotonics, Federal University of Bahia, 62, Araujo Pinho Ave, Canela, Salvador, BA, CEP 40110-150, Brazil. Electronic address: jscmonteiro@uefs.br., Rangel EE; Department of Biology, Feira de Santana State University, Feira de Santa, BA, CEP 44036-900, Brazil. Electronic address: erangell1.8@outlook.com., de Oliveira SCPS; Center of Biophotonics, Federal University of Bahia, 62, Araujo Pinho Ave, Canela, Salvador, BA, CEP 40110-150, Brazil. Electronic address: susampaiooliveira@gmail.com., Crugeira PJL; Center of Biophotonics, Federal University of Bahia, 62, Araujo Pinho Ave, Canela, Salvador, BA, CEP 40110-150, Brazil; Laboratory of Biotechnology and Ecology of Micro-organisms, Institute of Health Science, Federal University of Bahia, Reitor Miguel Calmon Ave, S/N, Salvador, BA, CEP:40110-100, Brazil. Electronic address: pedrocrugeira@yhaoo.com.br., Nunes IPF; Department of Biology, Feira de Santana State University, Feira de Santa, BA, CEP 44036-900, Brazil; Center of Biophotonics, Federal University of Bahia, 62, Araujo Pinho Ave, Canela, Salvador, BA, CEP 40110-150, Brazil. Electronic address: iago_fortuna@hotmail.com., de A Fagnani SRC; Department of Biology, Feira de Santana State University, Feira de Santa, BA, CEP 44036-900, Brazil; Center of Biophotonics, Federal University of Bahia, 62, Araujo Pinho Ave, Canela, Salvador, BA, CEP 40110-150, Brazil. Electronic address: sandrafagnani1@hotmail.com., Sampaio FJP; Department of Biology, Feira de Santana State University, Feira de Santa, BA, CEP 44036-900, Brazil; Center of Biophotonics, Federal University of Bahia, 62, Araujo Pinho Ave, Canela, Salvador, BA, CEP 40110-150, Brazil. Electronic address: fjpsam@icloud.com., de Almeida PF; Laboratory of Biotechnology and Ecology of Micro-organisms, Institute of Health Science, Federal University of Bahia, Reitor Miguel Calmon Ave, S/N, Salvador, BA, CEP:40110-100, Brazil. Electronic address: pfa@ufba.br., Pinheiro ALB; Center of Biophotonics, Federal University of Bahia, 62, Araujo Pinho Ave, Canela, Salvador, BA, CEP 40110-150, Brazil; National Institute of Basic Optics and Applied to Life Science, 400, Trabalhador São-Carlense Ave, Parque Arnold Schimidt, São Carlos, SP, CEP:13566-590, Brazil. Electronic address: albp@ufba.br.
Jazyk: angličtina
Zdroj: Photodiagnosis and photodynamic therapy [Photodiagnosis Photodyn Ther] 2020 Sep; Vol. 31, pp. 101930. Date of Electronic Publication: 2020 Jul 24.
DOI: 10.1016/j.pdpdt.2020.101930
Abstrakt: Photodynamic inactivation is a promising method for the treatment of infectious diseases. Nanotechnology through gold nanoparticles, as a tool to improve the delivery of photosensitizer is an attractive approach to enhance photodynamic inactivation of bacteria. Moreover, gold nanoparticles enchance the absorption of light due to their plasmon resonance. The aim of this study was to evaluate in vitro photodynamic inactivation effects of 1.9-Dimethyl-Methylene Blue (DMMB)-AuNPs associated with the red LED (λ630 ηm ± 20 ηm, 125 mW, 12 J / cm², 192 s) on S. aureus strain. Eight experimental groups were studied: Control, LED, AuNPs, AuNPs + LED, DMMB, DMMB + LED, DMMB + AuNPs, DMMB + AuNPs + LED. After incubation, the number of bacteria surviving each treatment was determined and then enumerated by viable counting (CFU / mL). The logarithm of CFU / mL (CFU/mL log 10 ) was calculated. All experiments realized in triplicate. The statistical analyses included one-way ANOVA tests, Tukey's multiple comparisons and nonlinear regression, p values <0.05 were considered statistically significant. According to results, the photodynamic inactivation of S. aureus on groups DMMB + LED and DMMB-AuNPs + LED, showed a significant reduction of the microbial load (p < 0.0001) when compared to the Control group. The decimal reduction (RD) of these groups were 99.96 % (RD = 3) and 99.994 % (RD = 4) respectively. In conclusion, these findings demonstrated that photodynamic inactivation is enhanced by using DMMB-AuNPs on S. aureus.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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