Enhancement in brain uptake of vitamin D 3 nanoemulsion for treatment of cerebral ischemia: formulation, gamma scintigraphy and efficacy study in transient middle cerebral artery occlusion rat models.

Autor: Kumar M; Department of Pharmaceutical Technology, Meerut Institute of Engineering & Technology, Meerut, India.; Department of Drug Development, Institute of Nuclear Medicine & Allied Sciences, DRDO (Ministry of Defence), New Delhi, India., Nishad DK; Department of Drug Development, Institute of Nuclear Medicine & Allied Sciences, DRDO (Ministry of Defence), New Delhi, India., Kumar A; Department of Pharmaceutical Technology, Meerut Institute of Engineering & Technology, Meerut, India., Bhatnagar A; Department of Drug Development, Institute of Nuclear Medicine & Allied Sciences, DRDO (Ministry of Defence), New Delhi, India., Karwasra R; Department of Drug Development, Institute of Nuclear Medicine & Allied Sciences, DRDO (Ministry of Defence), New Delhi, India., Khanna K; Department of Drug Development, Institute of Nuclear Medicine & Allied Sciences, DRDO (Ministry of Defence), New Delhi, India., S K; Department of Drug Development, Institute of Nuclear Medicine & Allied Sciences, DRDO (Ministry of Defence), New Delhi, India., Sharma D; Department of Drug Development, Institute of Nuclear Medicine & Allied Sciences, DRDO (Ministry of Defence), New Delhi, India., Dua K; Discipline of Pharmacy, Graduate School of Health, University of Technology Sydney, Ultimo, Australia.; Centre for Inflammation, Centenary Institute, Royal Prince Alfred Hospital, Sydney, Australia.; Priority Research Centre for Healthy Lungs, Hunter Medical Research Institute (HMRI) & School of Biomedical Sciences and Pharmacy, The University of Newcastle (UoN), Callaghan, Australia., Mudaliyar V; Department of Pharmaceutical Technology, Meerut Institute of Engineering & Technology, Meerut, India., Sharma N; Department of Pharmaceutical Technology, Meerut Institute of Engineering & Technology, Meerut, India.; Department of Drug Development, Institute of Nuclear Medicine & Allied Sciences, DRDO (Ministry of Defence), New Delhi, India.
Jazyk: angličtina
Zdroj: Journal of microencapsulation [J Microencapsul] 2020 Nov; Vol. 37 (7), pp. 492-501. Date of Electronic Publication: 2020 Aug 06.
DOI: 10.1080/02652048.2020.1801870
Abstrakt: Aim: For the treatment of cerebral ischaemia, vitamin-D 3 loaded nanoemulsions were developed.
Method: Tween 20 and polyethylene glycol were chosen as surfactant/co-surfactant, while oleic acid as the oil phase. The formulation was characterised for various in-vitro parameters. Targeting efficiency was investigated through radiometry, gamma scintigraphy and efficacy was studied in transient middle cerebral artery occlusion (MCAo) rat model.
Result: Vitamin D 3 -nanoemulsion showed a mean size range of 49.29 ± 10.28 nm with polydispersity index 0.17 ± 0.04 and zeta potential 13.77 mV. The formulation was found stable during thermodynamic stability study and permeated within 180 min through sheep nasal mucosa (permeation coefficient 7.873 ± 0.884 cm/h). Gamma scintigraphy and radiometry assay confirmed better percentage deposition (2.53 ± 0.17%) of 99m Tc-vitamin D 3 -nanoemulsion through nasal route compared to IV administered 99m Tc-vitamin D 3 solution (0.79 ± 0.03%). Magnetic Resonance Imaging (MRI) of the ischaemic model confirmed better efficacy of vitamin D 3 -nanoemulsion.
Conclusion: This work demonstrated better permeation, deposition, and efficacy of vitaminD 3 -nanoemulsion through the intranasal route.
Databáze: MEDLINE
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