A photocaged DNA nanocapsule for delivery and manipulation in cells.

Autor:	Feng Y; Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto, Japan., Tohgasaki T; FANCL Research Institute, Totsuka-ku, Yokohama, Japan., Shitomi Y; FANCL Research Institute, Totsuka-ku, Yokohama, Japan., Sugiyama H; Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto, Japan; Institute for Integrated Cell-Material Sciences, Kyoto University, Sakyo-ku, Kyoto, Japan. Electronic address: hs@kuchem.kyoto-u.ac.jp., Endo M; Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto, Japan; Institute for Integrated Cell-Material Sciences, Kyoto University, Sakyo-ku, Kyoto, Japan. Electronic address: endo@kuchem.kyoto-u.ac.jp.
Jazyk:	angličtina
Zdroj:	Methods in enzymology [Methods Enzymol] 2020; Vol. 641, pp. 329-342. Date of Electronic Publication: 2020 Jun 15.
DOI:	10.1016/bs.mie.2020.04.045
Abstrakt:	It is important to create new, specifically designed and controlled nanomaterials that can be used as molecular delivery systems for cells. Here, we describe a method for creating a nanosized DNA capsule (NC) using a photocaged unlocking system as a carrier for cell delivery. The photocaged NC (caged-NC) was designed and constructed to control the opening of the closed NC by photoirradiation. The opening of the NC was observed by atomic force microscopy, and the dynamic opening of the caged-NC was characterized by fluorescence quenching and recovery processes. The caged-NC was then introduced into the cytoplasm of a cell, where the photoinduced opening of the caged-NC was observed. The selective opening of the caged-NC in a single cell was successfully achieved by laser irradiation of individual cells. The caged-NC system could be used as a delivery system for relatively large nanomaterials in cells, similar to a native virus system. (© 2020 Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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