Status and Potential of Single-Cell Transcriptomics for Understanding Plant Development and Functional Biology.

Autor: Iqbal MM; UWA School of Agriculture and Environment, Faculty of Science, The University of Western Australia, 35 Stirling Hwy, Perth, WA, 6009, Australia.; Genome Innovation Hub, Telethon Kids Institute, Perth Children Hospital, Nedlands, WA, 6009, Australia., Hurgobin B; School of Life Sciences, La Trobe University, Bundoora, Victoria, 3086, Australia., Holme AL; Iain Fraser Cytometry Centre, IFCC Institute of Medical Sciences (IMS), School of Medicine, Medical Sciences and Nutrition University of Aberdeen Forester Hill, Aberdeen, AB25 2ZD, UK., Appels R; School of BioSciences, The University of Melbourne, Victoria, 3010, Australia.; School of Applied Biology, La Trobe University, Bundoora, Victoria, 3086, Australia., Kaur P; UWA School of Agriculture and Environment, Faculty of Science, The University of Western Australia, 35 Stirling Hwy, Perth, WA, 6009, Australia.
Jazyk: angličtina
Zdroj: Cytometry. Part A : the journal of the International Society for Analytical Cytology [Cytometry A] 2020 Oct; Vol. 97 (10), pp. 997-1006. Date of Electronic Publication: 2020 Aug 28.
DOI: 10.1002/cyto.a.24196
Abstrakt: The advent of modern "omics" technologies (genomics, transcriptomics, proteomics, and metabolomics) are attributed to innovative breakthroughs in genome sequencing, bioinformatics, and analytic tools. An organism's biological structure and function is the result of the concerted action of single cells in different tissues. Single cell genomics has emerged as a ground-breaking technology that has greatly enhanced our understanding of the complexity of gene expression at a microscopic resolution and holds the potential to revolutionize the way we characterize complex cell assemblies and study their spatial organization, dynamics, clonal distribution, pathways, function, and networking. Mammalian systems have benefitted immensely from these approaches to dissect complex systems such as cancer, immunological disorders, epigenetic controls of diseases, and understanding of developmental biology. However, the applications of single-cell omics in plant research are just starting. The potential to decipher the fundamentals of developmental and functional biology of large and complex plant species at the single-cell resolution are now becoming important drivers of research. In this review, we present the status, challenges and potential of one important and most commonly used single-cell omics technique in plants, namely single cell transcriptomics. © 2020 International Society for Advancement of Cytometry.
(© 2020 International Society for Advancement of Cytometry.)
Databáze: MEDLINE
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