| Autor: |
Zanchetta FC; School of Nursing, University of Campinas, Campinas CEP 13083887, Brazil., Trinca RB; Department of Engineering of Materials and of Bioprocess, School of Chemical Engineering, University of Campinas, Campinas CEP 13083852, Brazil., Gomes Silva JL; School of Nursing, University of Campinas, Campinas CEP 13083887, Brazil., Breder JDSC; School of Nursing, University of Campinas, Campinas CEP 13083887, Brazil., Cantarutti TA; School of Nursing, University of Campinas, Campinas CEP 13083887, Brazil., Consonni SR; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas, Campinas CEP 13083970, Brazil., Moraes ÂM; Department of Engineering of Materials and of Bioprocess, School of Chemical Engineering, University of Campinas, Campinas CEP 13083852, Brazil., Pereira de Araújo E; School of Nursing, University of Campinas, Campinas CEP 13083887, Brazil., Saad MJA; Department of Internal Medicine, University of Campinas, Campinas CEP 13083887, Brazil., Adams GG; School of Health Sciences, Faculty of Medicine, The University of Nottingham, C Floor, South Block Link, Queen's Medical Centre, Nottingham NG7 2HA, UK., Melo Lima MH; School of Nursing, University of Campinas, Campinas CEP 13083887, Brazil. |
| Abstrakt: |
Polycaprolactone (PCL) is a synthetic polymer with good mechanical properties that are useful to produce biomaterials of clinical application. It can be successfully combined with chitosan, which enhances the biomaterial properties through the modulation of molecular and cellular mechanisms. The objective of this study was to evaluate the effects of the use of electrospun fibrous membranes consisting of polycaprolactone (PCL) or polycaprolactone coated with chitosan and poly(ethylene oxide) (PCL+CHI/PEO) on mouse skin lesions. Sixty four Black-57 mice were divided into PCL and PCL+CHI/PEO groups. A 1 cm 2 lesion was made on the animals' backs, and the membranes were sutured in place. The tissues were extracted on the 3rd, 7th, and 14th days after the lesion. The tissues were analyzed by histology with Hematoxylin and Eosin (H&E) and Sirius Red stains, morphometry, immunohistochemistry, and Western blot. On the 3rd, 6th, and 9th days after the lesion, the PCL+CHI/PEO group showed a higher wound-healing rate (WHR). On the 3 day, the PCL+CHI/PEO group showed a greater amount of inflammatory infiltrate, greater expression of proliferating cell nuclear antigen (PCNA), and smooth muscle actin (α-SMA) ( p < 0.05) compared to the PCL group. On the 7th day after the lesion, the PCL+CHI/PEO group showed a greater amount of inflammatory infiltrate, expression of Tumor Necrosis Factor (TNF-α) and PCNA ( p < 0.05). In addition, it showed a greater immunolabeling of Monocyte Chemoattractant Protein-1 (MCP-1) and deposition of collagen fibers compared to the PCL group. The PCL+CHI/PEO membrane modulated the increase in the inflammatory infiltrate, the expression of MCP-1, PCNA, and α-SMA in lesions of mice. |
