Characterization of mammalian orthoreoviruses isolated from faeces of pigs in Zambia.

Autor:	Harima H; Hokudai Center for Zoonosis Control in Zambia, School of Veterinary Medicine, the University of Zambia, PO Box 32379, Lusaka, 10101, Zambia., Sasaki M; Division of Molecular Pathobiology, Hokkaido University, Research Center for Zoonosis Control, N20 W10, Kita-ku, Sapporo, 001-0020, Japan., Kajihara M; Division of Global Epidemiology, Hokkaido University, Research Center for Zoonosis Control, N20 W10, Kita-ku, Sapporo, 001-0020, Japan.; Hokudai Center for Zoonosis Control in Zambia, School of Veterinary Medicine, the University of Zambia, PO Box 32379, Lusaka, 10101, Zambia., Gonzalez G; Division of Bioinformatics, Hokkaido University, Research Center for Zoonosis Control, N20 W10, Kita-ku, Sapporo, 001-0020, Japan., Simulundu E; Department of Disease Control, School of Veterinary Medicine, the University of Zambia, PO Box 32379, Lusaka, 10101, Zambia., Bwalya EC; Department of Clinical Studies, School of Veterinary Medicine, the University of Zambia, PO Box 32379, Lusaka, 10101, Zambia., Qiu Y; Hokudai Center for Zoonosis Control in Zambia, School of Veterinary Medicine, the University of Zambia, PO Box 32379, Lusaka, 10101, Zambia., Okuya K; Division of Global Epidemiology, Hokkaido University, Research Center for Zoonosis Control, N20 W10, Kita-ku, Sapporo, 001-0020, Japan., Isono M; Division of Global Epidemiology, Hokkaido University, Research Center for Zoonosis Control, N20 W10, Kita-ku, Sapporo, 001-0020, Japan., Orba Y; Division of Molecular Pathobiology, Hokkaido University, Research Center for Zoonosis Control, N20 W10, Kita-ku, Sapporo, 001-0020, Japan., Takada A; Division of Global Epidemiology, Hokkaido University, Research Center for Zoonosis Control, N20 W10, Kita-ku, Sapporo, 001-0020, Japan.; Department of Disease Control, School of Veterinary Medicine, the University of Zambia, PO Box 32379, Lusaka, 10101, Zambia.; Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University Kita-ku, Sapporo, 001-0020, Japan.; Africa Center of Excellence for Infectious Diseases of Humans and Animals, the University of Zambia, PO Box 32379, Lusaka, 10101, Zambia., Hang'ombe BM; Department of Para-clinical Studies, School of Veterinary Medicine, the University of Zambia, PO Box 32379, Lusaka, 10101, Zambia.; Africa Center of Excellence for Infectious Diseases of Humans and Animals, the University of Zambia, PO Box 32379, Lusaka, 10101, Zambia., Mweene AS; Africa Center of Excellence for Infectious Diseases of Humans and Animals, the University of Zambia, PO Box 32379, Lusaka, 10101, Zambia.; Department of Disease Control, School of Veterinary Medicine, the University of Zambia, PO Box 32379, Lusaka, 10101, Zambia., Sawa H; Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University Kita-ku, Sapporo, 001-0020, Japan.; Department of Disease Control, School of Veterinary Medicine, the University of Zambia, PO Box 32379, Lusaka, 10101, Zambia.; Global Virus Network, Baltimore, Maryland, 21201, 725 West Lombard St, Room S413, Baltimore, USA.; Division of Molecular Pathobiology, Hokkaido University, Research Center for Zoonosis Control, N20 W10, Kita-ku, Sapporo, 001-0020, Japan.; Africa Center of Excellence for Infectious Diseases of Humans and Animals, the University of Zambia, PO Box 32379, Lusaka, 10101, Zambia.
Jazyk:	angličtina
Zdroj:	The Journal of general virology [J Gen Virol] 2020 Oct; Vol. 101 (10), pp. 1027-1036. Date of Electronic Publication: 2020 Jul 24.
DOI:	10.1099/jgv.0.001476
Abstrakt:	Mammalian orthoreovirus (MRV) has been identified in humans, livestock and wild animals; this wide host range allows individual MRV to transmit into multiple species. Although several interspecies transmission and genetic reassortment events of MRVs among humans, livestock and wildlife have been reported, the genetic diversity and geographic distribution of MRVs in Africa are poorly understood. In this study, we report the first isolation and characterization of MRVs circulating in a pig population in Zambia. In our screening, MRV genomes were detected in 19.7 % (29/147) of faecal samples collected from pigs by reverse transcription PCR. Three infectious MRV strains (MRV-85, MRV-96 and MRV-117) were successfully isolated, and their complete genomes were sequenced. Recombination analyses based on the complete genome sequences of the isolated MRVs demonstrated that MRV-96 shared the S3 segment with a different MRV isolated from bats, and that the L1 and M3 segments of MRV-117 originated from bat and human MRVs, respectively. Our results suggest that the isolated MRVs emerged through genetic reassortment events with interspecies transmission. Given the lack of information regarding MRVs in Africa, further surveillance of MRVs circulating among humans, domestic animals and wildlife is required to assess potential risk for humans and animals.
Databáze:	MEDLINE
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