Hyptis suaveolens (L.) Poit protects colon from TNBS-induced inflammation via immunomodulatory, antioxidant and anti-proliferative mechanisms.

Autor: Machado FDF; Postgraduate Program in Natural and Synthetic Bioactive Products, Health Sciences Center, Universidade Federal da Paraíba (UFPB), João Pessoa, PB, Brazil. Electronic address: flaviadanniele@hotmail.com., Formiga RO; Postgraduate Program in Natural and Synthetic Bioactive Products, Health Sciences Center, Universidade Federal da Paraíba (UFPB), João Pessoa, PB, Brazil. Electronic address: rodrigo.formiga@hotmail.com., Lima GRM; Postgraduate Program in Natural and Synthetic Bioactive Products, Health Sciences Center, Universidade Federal da Paraíba (UFPB), João Pessoa, PB, Brazil. Electronic address: gedsonfarm@hotmail.com., de Jesus NZT; Postgraduate Program in Natural and Synthetic Bioactive Products, Health Sciences Center, Universidade Federal da Paraíba (UFPB), João Pessoa, PB, Brazil. Electronic address: neyresj@hotmail.com., Alves Júnior EB; Postgraduate Program in Natural and Synthetic Bioactive Products, Health Sciences Center, Universidade Federal da Paraíba (UFPB), João Pessoa, PB, Brazil. Electronic address: edvaldojunioralves@gmail.com., Marinho AF; Postgraduate Program in Natural and Synthetic Bioactive Products, Health Sciences Center, Universidade Federal da Paraíba (UFPB), João Pessoa, PB, Brazil. Electronic address: alexsandromarinho@ltf.ufpb.br., Tavares JF; Postgraduate Program in Natural and Synthetic Bioactive Products, Health Sciences Center, Universidade Federal da Paraíba (UFPB), João Pessoa, PB, Brazil. Electronic address: josean@ltf.ufpb.br., Santos FA; Laboratory of Natural Products, Universidade Federal do Ceará (UFC), Fortaleza, CE, Brazil. Electronic address: fasufc@gmail.com., Viana AFSC; Laboratory of Natural Products, Universidade Federal do Ceará (UFC), Fortaleza, CE, Brazil. Electronic address: flavia_seraine@hotmail.com., Araújo AA; Department of Biophysics and Pharmacology, Universidade Federal do Rio Grande Norte (UFRN), Natal, RN, Brazil. Electronic address: aurigena@ufrnet.br., de Araújo Júnior RF; Department of Morphology, Histology and Basic Pathology, Federal University of Rio Grande do Norte, Natal, RN, Brazil. Electronic address: araujojr@cb.ufrn.br., Pellizzon CH; Laboratory of Histology UNIPEX-FMB, Universidade de São Paulo (UNESP), Botucatu, SP, Brazil. Electronic address: claudia.pellizzon@gmail.com., Batista LM; Postgraduate Program in Natural and Synthetic Bioactive Products, Health Sciences Center, Universidade Federal da Paraíba (UFPB), João Pessoa, PB, Brazil. Electronic address: leoniab@uol.com.br.
Jazyk: angličtina
Zdroj: Journal of ethnopharmacology [J Ethnopharmacol] 2021 Jan 30; Vol. 265, pp. 113153. Date of Electronic Publication: 2020 Jul 20.
DOI: 10.1016/j.jep.2020.113153
Abstrakt: Ethnopharmacological Relevance: In folk medicine Hyptis suaveolens (Lamiaceae) has been reported to relieve respiratory and gastrointestinal infections, indigestion, cold, pain, fever, cramps, skin diseases, gastric ulcer and inflammatory disorders. This study investigated the effects and the mechanisms of action of Hyptis suaveolens (L.) Poit (Lamiaceae) ethanol extract (Hs-EtOH) and hexane phase (Hs-HexF) against intestinal inflammation.
Material and Methods: Acute and relapse TNBS-induced ulcerative colitis protocols were used to evaluate intestinal anti-inflammatory activity. Damage evaluations, biochemical, histological and immunostaining parameters were determined.
Results: Both extracts decreased macroscopic colonic inflammation and the area of lesion induced by TNBS. Nevertheless, only Hs-HexF was able to reduce colonic wall thickness, edema and diffuse inflammatory cell infiltration and to prevent GSH depletion in the acute model of ulcerative colitis. In the chronic phase with relapse of colonic ulceration, yet again only Hs-HexF significantly attenuated inflammatory parameters and presented a decrease in nitrite/nitrate, MDA, MPO, IL-1-β and TNF-α and increased levels of SOD, CAT, GSH and IL-10. Hs-HexF also significantly reduced positive cells immunostained for PCNA.
Conclusion: The data indicate intestinal anti-inflammatory activity for H. suaveolens, due to the participation of the antioxidant system, decreased neutrophil infiltration and cytokine modulation, as well as, owing to regulation of cell proliferation.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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