| Autor: |
El-Bendary MA; Department of Microbial Chemistry, Genetic Engineering and Biotechnology Research Division, National Research Centre, Giza, Egypt., Afifi SS; Department of Microbiology and Immunology, Faculty of Pharmacy for Girls, Al-Azhar University, Cairo, Egypt., Moharam ME; Department of Microbial Chemistry, Genetic Engineering and Biotechnology Research Division, National Research Centre, Giza, Egypt., Abo El-Ola SM; Department of Protein and Manmade Fibre, Textile Industries Research Division, National Research Centre, Giza, Egypt., Salama A; Department of Pharmacology, National Research Centre, Giza, Egypt., Omara EA; Department of Pathology, National Research Centre, Giza, Egypt., Shaheen MNF; Environmental Virology Lab., Department of Water Pollution Research, Environmental Research Division, National Research Centre, Giza, Egypt., Hamed AA; Department of Microbial Chemistry, Genetic Engineering and Biotechnology Research Division, National Research Centre, Giza, Egypt., Gawdat NA; Department of Microbial Chemistry, Genetic Engineering and Biotechnology Research Division, National Research Centre, Giza, Egypt. |
| Abstrakt: |
Silver nanoparticles (AgNPs) have unique properties and a large range of applications. Biosynthesis of stable AgNPs using the extracellular filtrate of Bacillus subtilis was proved by the characteristic surface plasmon resonance at about 420-430 nm. They were polycrystalline with spherical, hexagonal, and irregular shapes and they were negatively charged (-40 mV) with an average diameter of 20 nm. FTIR spectrum confirmed the presence of protein molecules coating AgNPs. The optimum conditions for the synthesis of tested AgNPs were 1:6 filtrate dilution, 1 mM AgNO 3 , pH 7, 30 °C , 48 h contact time under static and illuminating conditions. The synthesized AgNPs showed antibacterial activities against Escherichia coli , Staphylococcus aureus , Pseudomonas aeruginosa , Bacillus cereus , and Salmonella typhi , antifungal activity against Candida albicans and antiviral activity against rotavirus. Also, they showed potent cytotoxic effects on lung and hepatic carcinoma human cell lines. Meanwhile, the acute toxicity study against mice showed no significant changes in hematological, biochemical, and histological parameters of AgNPs treated mice. They also showed mild hepatoprotective effect in thioacetamide (TAA) - induced hepatic fibrosis in rats. AgNPs treated textiles fabrics showed high antimicrobial activities against different pathogenic microorganisms as well as UV protection adequacy. |
