Convergent Chemoenzymatic Strategy to Deliver a Diversity of Shigella flexneri Serotype-Specific O-Antigen Segments from a Unique Lightly Protected Tetrasaccharide Core.

Autor: Hu Z; Unité de Chimie des Biomolécules, Institut Pasteur, UMR3523 CNRS, 28 Rue du Dr Roux, 75724 Paris Cedex 15, France., Benkoulouche M; Toulouse Biotechnology Institute, TBI, Université de Toulouse, CNRS, INRA, INSA, Toulouse, France. 135, Avenue de Rangueil, F-31077 Toulouse Cedex 04, France., Barel LA; Unité de Chimie des Biomolécules, Institut Pasteur, UMR3523 CNRS, 28 Rue du Dr Roux, 75724 Paris Cedex 15, France.; Université Paris Descartes, Sorbonne Paris Cité, 75006 Paris, France., Le Heiget G; Unité de Chimie des Biomolécules, Institut Pasteur, UMR3523 CNRS, 28 Rue du Dr Roux, 75724 Paris Cedex 15, France.; Université Paris 13, Sorbonne Paris Cité, 93430 Paris, France., Ben Imeddourene A; Toulouse Biotechnology Institute, TBI, Université de Toulouse, CNRS, INRA, INSA, Toulouse, France. 135, Avenue de Rangueil, F-31077 Toulouse Cedex 04, France., Le Guen Y; Unité de Chimie des Biomolécules, Institut Pasteur, UMR3523 CNRS, 28 Rue du Dr Roux, 75724 Paris Cedex 15, France.; Université Paris Descartes, Sorbonne Paris Cité, 75006 Paris, France., Monties N; Toulouse Biotechnology Institute, TBI, Université de Toulouse, CNRS, INRA, INSA, Toulouse, France. 135, Avenue de Rangueil, F-31077 Toulouse Cedex 04, France., Guerreiro C; Unité de Chimie des Biomolécules, Institut Pasteur, UMR3523 CNRS, 28 Rue du Dr Roux, 75724 Paris Cedex 15, France., Remaud-Siméon M; Toulouse Biotechnology Institute, TBI, Université de Toulouse, CNRS, INRA, INSA, Toulouse, France. 135, Avenue de Rangueil, F-31077 Toulouse Cedex 04, France., Moulis C; Toulouse Biotechnology Institute, TBI, Université de Toulouse, CNRS, INRA, INSA, Toulouse, France. 135, Avenue de Rangueil, F-31077 Toulouse Cedex 04, France., André I; Toulouse Biotechnology Institute, TBI, Université de Toulouse, CNRS, INRA, INSA, Toulouse, France. 135, Avenue de Rangueil, F-31077 Toulouse Cedex 04, France., Mulard LA; Unité de Chimie des Biomolécules, Institut Pasteur, UMR3523 CNRS, 28 Rue du Dr Roux, 75724 Paris Cedex 15, France.
Jazyk: angličtina
Zdroj: The Journal of organic chemistry [J Org Chem] 2021 Feb 05; Vol. 86 (3), pp. 2058-2075.
DOI: 10.1021/acs.joc.0c00777
Abstrakt: Progress in glycoscience is strongly dependent on the availability of broadly diverse tailor-made, well-defined, and often complex oligosaccharides. Herein, going beyond natural resources and aiming to circumvent chemical boundaries in glycochemistry, we tackle the development of an in vitro chemoenzymatic strategy holding great potential to answer the need for molecular diversity characterizing microbial cell-surface carbohydrates. The concept is exemplified in the context of Shigella flexneri , a major cause of diarrhoeal disease. Aiming at a broad serotype coverage S. flexneri glycoconjugate vaccine, a non-natural lightly protected tetrasaccharide was designed for compatibility with (i) serotype-specific glucosylations and O -acetylations defining S. flexneri O-antigens, (ii) recognition by suitable α-transglucosylases, and (iii) programmed oligomerization following enzymatic α-d-glucosylation. The tetrasaccharide core was chemically synthesized from two crystalline monosaccharide precursors. Six α-transglucosylases found in the glycoside hydrolase family 70 were shown to transfer glucosyl residues on the non-natural acceptor. The successful proof of concept is achieved for a pentasaccharide featuring the glucosylation pattern from the S. flexneri type IV O-antigen. It demonstrates the potential of appropriately planned chemoenzymatic pathways involving non-natural acceptors and low-cost donor/transglucosylase systems to achieve the demanding regioselective α-d-glucosylation of large substrates, paving the way to microbial oligosaccharides of vaccinal interest.
Databáze: MEDLINE
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